| 三氧化二砷诱导大鼠血管平滑肌细胞凋亡的实验研究 |
| |
| 引用本文： | 邵建伟,华尔铨,吴宗贵,李莉,张玲珍,仲人前,孔宪涛. 三氧化二砷诱导大鼠血管平滑肌细胞凋亡的实验研究[J]. 中国药学杂志, 2002, 37(12): 910-913 |
| |
| 作者姓名： | 邵建伟 华尔铨 吴宗贵 李莉 张玲珍 仲人前 孔宪涛 |
| |
| 作者单位： | 1. 上海杨浦区中心医院心内科,上海,200090
2. 第二军医大学上海长征医院,上海,200003 |
| |
| 摘 要： | 目的：探讨三氧化二砷抗动脉粥样硬化的效应及机制。方法：建立大鼠主动脉血管平滑肌增殖模型。通过四氮唑蓝还原反应、免疫组化、Western 印迹观察三氧化二砷作用后细胞凋亡和诱导机制。结果：免疫组化、Western 印迹显示随三氧化二砷浓度的增高及作用时间的延长，Bcl-2蛋白表达下降。结论：三氧化二砷诱导VSMCs凋亡的机制可能是通过下调过度表达的Bcl-2蛋白所致。
|
| 关 键 词： | 细胞凋亡 三氧化二砷 血管平滑肌细胞 Bcl－2 动脉粥样硬化 动物模型 |
| 文章编号： | 1001-2494(2002)12-0910-04 |
| 修稿时间： | 2001-12-12 |
Experimental study of vascular smooth muscle cell apoptosis induced by arsenic trioxide in rats |
| |
| SHAO Jian wei ,HUA Er quan ,WU Zong gui ,LI Li ,Z HANG Ling zhen ,ZHONG Ren qian ,KONG Xian tao. Experimental study of vascular smooth muscle cell apoptosis induced by arsenic trioxide in rats[J]. Chinese Pharmaceutical Journal, 2002, 37(12): 910-913 |
| |
| Authors: | SHAO Jian wei HUA Er quan WU Zong gui LI Li Z HANG Ling zhen ZHONG Ren qian KONG Xian tao |
| |
| Affiliation: | SHAO Jian wei 1,HUA Er quan 1,WU Zong gui 2,LI Li 2,Z HANG Ling zhen 2,ZHONG Ren qian 2,KONG Xian tao 2 |
| |
| Abstract: | OBJECTIVE To investigate the antiatherogenetic e ff ect of arsenic trioxide and its mechanisms.METHODS VSMCs pro liferation model of cultured rat thoracic aorta was established. NBT reduction t est, immunohistochemical staining, western blot were applied respectively to obs erve the apoptotic changes.RESULTS The results of immunohist ochemical staining and western blot showed the expression of Bcl 2 decreased in concentration and time dependency.CONCLUSION Arsenic trio xide can induce apoptosis of rat proliferated VSMCs,and this mechanism maybe the expression of Bcl 2 protein downregulated. |
| |
| Keywords: | arsenic trioxide vascular smooth muscle cells Bcl 2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
