HPLC同时测定大血藤中红景天苷和绿原酸的含量

目的建立高效液相转换波长法,同时测定大血藤中红景天苷和绿原酸的含量。方法采用Inertsil ODS-SP色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水溶液梯度洗脱;流速1 mL.min 1,柱温30℃,检测波长220,325 nm。结果红景天苷和绿原酸分别在0.061 7～1.234μg(r=0.999 7)和0.030 2～0.605μg(r=0.999 9)与峰面积呈良好的线性关系,平均回收率(n=6)分别为97.8%和98.1%,RSD分别为1.9%和1.7%。结论该方法同时测定大血藤中的红景天苷和绿原酸的含量,方法简单、准确度高、重复性好,可为大血藤药材的质量控制提供参考。

Simultaneous Determination of Salidroside and Chlorogenic Acid in Sargentodoxa Cueata by HPLC

OBJECTIVE To establish a wavelength conversion method for determination of salidroside and chlorogenicacid in Sargentodoxa cueata. METHODS The separation was performed on a Inertsil ODS-SP column（4.6 minx250 mm, 5 μm）. Acetonitril-0.1% H3PO4 was used as the mobile phase with gradient elution. The flow rate was 1 mL.min-1. The detection wavelength was 220 nm and 325 nm. RESULTS The linear range was 0.061 7-1.234 p.g（r=0.999 7） and 0.030 2-0.605 μg （r=0.999 9）, respectively. The average recoveries（n=6） of salidroside and chlorogenic acid were 97.8% and 98.1%, respectively. CONCLUSION This method is simple, sensitive and it may be used for the quality control of Sargentodoxa cuneata.