应用PCR技术研究真菌在鼻息肉及鼻窦炎发病中的作用

目的：探讨真菌在慢性鼻窦炎、鼻息肉患者中的致病作用。方法：采用PCR方法分别检测66例慢性鼻窦炎患者（鼻窦炎组）生理盐水冲洗前、后的鼻息肉及鼻窦黏膜的真菌，同时检测21例正常志愿者（对照组）的鼻腔分泌物中的真菌，应用Y。检验比较其差异性。结果：鼻息肉组真菌阳性率：息肉为89．2％，黏膜为89．4％，分别与对照组（66．7％）比较，差异均有统计学意义（均P〈0.05）；用生理盐水冲洗后，鼻息肉组真菌阳性率：息肉为7．14％，黏膜为2．63％，分别与冲洗前比较，差异均有统计学意义（均P〈0.05）。结论：真菌在鼻息肉和鼻窦炎的发病中可能发挥重要作用，PCR技术在真菌检测中有重要意义。

The effect of fungi in the pathogenesis of nasal polyps and chronic rhinosinusitis

OBJECTIVE: To study the effect of fungi in the pathogenesis of nasal polyps and chronic rhinosinusitis (CRS). METHOD: This study were divided two groups, the first was the experimental group: Nasal mucus membrane and nasal polyps were collected from patients with CRS (with and without polyps), and the second was the control group, nasal mucus were collected from OHc. Fungal-specific PCR were performed on each sample. The anther was used 0.9% sol sodium chloride to wash the autopsy tissues 5 times, and then PCR analysis was performed. chi-squared analysis was used to test for statistical difference between these groups. RESULT: PCR technique detected fungal DNA were 89.2% and 89.4% in nasal polyps and nasal mucous membrane respectively, and 66.7% in normal control subjective. There were satistically significant difference in these groups. After using 0.9% sol sodium chloride to wash the autopsy tissues, the fungi's positive rate was droped. CONCLUSION: Fungi was important in the pathogenesis of nasal polyps and CRS.