| 旋毛虫新生幼虫T668重组抗原对小鼠的保护性免疫 |
| |
| 引用本文： | 牛廷献,刘明远,卢强,付宝权,Pascal Boireau. 旋毛虫新生幼虫T668重组抗原对小鼠的保护性免疫[J]. 免疫学杂志, 2005, 21(1): 20-22 |
| |
| 作者姓名： | 牛廷献 刘明远 卢强 付宝权 Pascal Boireau |
| |
| 作者单位： | 兰州军区总医院动物实验科,兰州,730050;解放军军需大学军事兽医系,长春,130062;法国食品卫生安全局,巴黎 94703 |
| |
| 基金项目： | 国家自然科学基金 (30 1 70 70 9)，中法先进计划项目(961 2 )资助 |
| |
| 摘 要： | 目的研究旋毛虫新生幼虫T668重组抗原的免疫原性，制备基因工程疫苗。方法以旋毛虫新生幼虫期特异性基因T668在大肠杆菌中的表达蛋白为抗原免疫小鼠，每间隔10d免疫1次，共免疫3次。末次免疫后10d，每只小鼠攻击感染200条旋毛虫感染性肌幼虫，感染后5周用消化法检查肌幼虫(ML)负荷。结果T668重组抗原免疫组肌幼虫减虫率明显高于佐剂组和对照组。结论T668重组抗原能诱导小鼠产生一定程度的保护性免疫，且可激发特异性体液免疫。
|
| 关 键 词： | 旋毛虫新生幼虫 重组抗原 保护性免疫 |
| 文章编号： | 1000-8861(2005)01-0020-03 |
| 修稿时间： | 2004-02-17 |
Studies on protective immunity of Trichinella spiralis newborn larvae recombinant antigen in mice |
| |
| NIU Ting-xian ,LIU Ming-yuan ,LU Qiang ,FU Bao-quan ,Pascal Boireau. Studies on protective immunity of Trichinella spiralis newborn larvae recombinant antigen in mice[J]. Immunological Journal, 2005, 21(1): 20-22 |
| |
| Authors: | NIU Ting-xian LIU Ming-yuan LU Qiang FU Bao-quan Pascal Boireau |
| |
| Affiliation: | NIU Ting-xian 1,LIU Ming-yuan 2,LU Qiang 2,FU Bao-quan 2,Pascal Boireau 3 |
| |
| Abstract: | Objective To study the immunogenicity of Trichinella spiralis (T.s) newborn larvae (NBL) recombinant antigen, and to produce genic engineer vaccine. Methods The NBL stage-specific gene T668 was expressed in E.coli and the expression protein was used to immunize the mice at an interval of 10 days. Ten days after the final immunization, the animals were challenged with 200 T.s infective muscle larvae (ML) per mice. ML loading was detected by digest method at week 5 after the challenge infection. Results The reduction rate of ML in immunized group was significantly higher than that in the adjuvant and control group. Conclusion T668 recombinant antigen could induce protective immunity as well as specific humoral immunity. |
| |
| Keywords: | Trichinella spiralis Recombinant antigen Protective immunity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
