纳米羟基磷灰石人工骨修复骨缺损的实验研究

目的：探讨多孔Nano-Hydmxyapatite（Nano-HA）人工骨的骨缺损修复作用及相关问题，为临床骨缺损修复提供依据。方法：采用新西兰大白兔39只，单侧桡骨制备骨缺损动物模型，用多孔Nano-HA人工骨材料植入骨缺损处进行修复作为实验组，以HA人工骨材料及空白组作为对照组；术后4、8、12周分别行大体标本观察、组织学、X线、扫描电镜及生物力学测试，综合评价多孔Nano-HA人工骨对骨缺损的修复能力及对机体的影响。结果：Nano-HA人工骨和HA人工骨均可促进新骨形成，前者新骨形成量大，骨缺损修复能力明显优于后者，差异有显著性（P〈0．05）。结论：Nano-HA人工骨具有良好的成骨能力和生物相容性，可望成为一种理想的骨缺损修复材料。

Experimental study of the nano-hydroxyapatite artificial bone in repairing the bone defect

Objective: To study the ability of nano-hydroxyapatite artificial bone in repairing and reconstructing of bone defect and the correlated problems. Methods: The animal model of bone defect was made on the bilateral radius of 39 New Zealand white rabbits, which were then divided into experimental group(the bone defect was repaired with nano-HA artificial bone), control group(the bone defect was repaired with HA artificial bone), bland group (the defect was left unrepaired)randomly. The reconstructive ability of bone defect was evaluated by gross observation, histopathological study, X-ray examination, SEM and biomechanical analysis at the 4th,8th and 12th week postoperatively. Results: The experimental group stimulated more bone formation than that of the control and the bland groups. The difference of reconstructive ability was statistically significant(P<0.05). Conclusions: The Nano-HA artificial bone is favourable in repair ability and biocompatibility,it can be used in the treatment of bone defect.