| 液-质联用法初步研究健康人血浆内源性还原型谷胱甘肽的昼夜差异及饮食影响 |
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| 引用本文： | 张全英,华雯妍,朱艺芳,黄明,王蒙,施爱明,周文佳.液-质联用法初步研究健康人血浆内源性还原型谷胱甘肽的昼夜差异及饮食影响[J].中国医院药学杂志,2011(3). |
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| 作者姓名： | 张全英 华雯妍 朱艺芳 黄明 王蒙 施爱明 周文佳 |
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| 作者单位： | 苏州大学附属第二医院临床药理实验室; |
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| 基金项目： | 江苏省卫生厅医学基金临床药学科研课题(编号:200704) |
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| 摘 要： | 目的:初步研究时辰及饮食对健康中国人血浆中内源性还原型谷胱甘肽(GSH)的影响。方法:16名男性健康受试者分别在不同时辰、餐前及餐后采集静脉血2mL,以已建立的HPLC-MS/MS快速测定法测定血浆中内源性GSH的浓度,数据使用SPSS软件统计分析,比较不同时辰以及餐前餐后血浆中内源性GSH浓度差异有无显著性。结果:全部测定在一批内完成,16名男性健康受试者早晨空腹、早晨餐后和晚上空腹的血浆GSH浓度分别为(0.18±0.06),(0.23±0.11),(0.12±0.04)mg.L-1。3组数据两两配对t检验结果P值均小于0.05,具有显著性差异。结论:初步研究显示时辰和饮食对血浆中内源性GSH浓度影响均有显著性。
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| 关 键 词： | 还原性谷胱甘肽 液-质联用 血浆浓度 昼夜节律 饮食影响 |
Preliminary study of the effect circadian rhythm and food on the concentration of endogenous reduced glutathione in plasma of healthy Chinese by HPLC-MS/MS |
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| ZHANG Quan-ying,HUA Wen-yan,ZHU Yi-fang,HUANG Ming,WANG Meng,SHI Ai-ming,ZHOU Wen-jia.Preliminary study of the effect circadian rhythm and food on the concentration of endogenous reduced glutathione in plasma of healthy Chinese by HPLC-MS/MS[J].Chinese Journal of Hospital Pharmacy,2011(3). |
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| Authors: | ZHANG Quan-ying HUA Wen-yan ZHU Yi-fang HUANG Ming WANG Meng SHI Ai-ming ZHOU Wen-jia |
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| Institution: | ZHANG Quan-ying,HUA Wen-yan,ZHU Yi-fang,HUANG Ming,WANG Meng,SHI Ai-ming,ZHOU Wen-jia (Clinical Pharmacology Laboratory,The Second Affiliate Hospital of Soochow University,Jiangsu Suzhou 215004,China) |
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| Abstract: | OBJECTIVE To study the effect of circadian rhythm and food on the concentration of endogenous GSH in plasma of healthy Chinese volunteers.METHODS The plasma samples of sixteen healthy male volunteers were collected fasting in the morning,after meal in the morning and fasting in the evening.All the samples were determined by HPLC-MS/MS method which has been validated.All of the concentration data were statistic by the SPSS statistical soft to analysis if the concentrations were significant deviation.RESULTS ... |
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| Keywords: | reduced glutathione HPLC-MS/MS plasma concentration circadian rhythm food effect |
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