胃肠道恶性肿瘤患者CD4+CD25+调节性T细胞的检测及意义

目的探讨胃肠道恶性肿瘤患者外周血及病理组织调节性T细胞(Tregs)的表达及临床意义。方法采用流式细胞仪对60例胃肠道恶性肿瘤患者及20例健康对照者外周血CD4+CD25+Foxp3+T细胞占CD4+T细胞(CD4+CD25+Foxp3+Tregs/CD4+T)的比率进行检测,采用免疫组化法检测相应患者病理组织中CD4+CD25+Foxp3+Tregs的表达水平。结果胃癌、结直肠癌患者外周血CD4+CD25+Foxp3+Tregs/CD4+T细胞的比率分别为(7.46±0.85)%和(7.79±1.19)%,与对照组(6.65±0.79)%比较,差异有统计学意义(P<0.01)。胃癌、结直肠癌病理组织中CD4+CD25+Foxp3+Tregs的表达平均阳性细胞绝对数为(53.52±16.31)和(58.15±14.86),与癌旁组织(6.53±2.68)比较,差异有统计学意义(P<0.01)。结论胃癌、结直肠癌患者外周血及病理组织中Tregs的高表达可能具有肿瘤免疫的抑制作用,并与肿瘤的发生、发展密切相关。

Expression and clinical significance of CD4~+ CD25~+ Tregs in patients with gastrointestinal malignancy

Objective To study the expression of CD4+CD25+ regulatory T cells(Tregs) in peripheral blood and pathological tissue of patients with gastrointestinal malignancy.Methods The proportion of CD4+CD25+Foxp3+Tregs/CD4+T in peripheral blood was determined with flow cytometry in 60 patients with gastrointestinal malignancy and 20 healthy controls.The expression of CD4+CD25+Foxp3+ Tregs was evaluated by immunohistochemical method in carcinoma tissue and adjacent noncancerous tissue from the patients.Results The proportion of CD4+CD25+Fop3+Tregs/CD4+T in peripheral blood from patients with gastric cancer and colorectal cancer accounted for 7.46%±0.85% and 7.79%±1.19% respectively,and were both significantly higher than those of healthy controls(6.65±0.79)%(P<0.01).The average absolute numbers of positive Foxp3+Tregs in carcinoma tissue from patients with gastric cancer and colorectal cancer were 53.52±16.31 and 58.15±14.86 per square millimeter,and were both significantly higher than those of adjacent noncancerous tissue(6.53±2.68)/mm2(P<0.01).Conclusion The increase percent of Tregs may be closely related with immunosuppression and tumor progression in patients with gastrointestinal malignancy.