维吾尔族妇女宫颈癌发生与EBV及HPV感染的关系

目的:探讨维吾尔族妇女宫颈癌发生过程中EB病毒(Ebstein-Barr virus,EBV)及人乳头瘤病毒(human papillomavirus,HPV)感染的作用及其意义。方法:收集维吾尔族妇女宫颈炎、宫颈内上皮瘤样病变(cervical intraepithelial neoplasja,CIN)Ⅰ/Ⅱ/Ⅲ和宫颈鳞癌患者福尔马林浸泡与石蜡包埋组织标本共178例,提取DNA并采用PCR方法对EBV和HPV DNA进行检测;用免疫组织化学方法检测宫颈癌EBV蛋白表达。结果:病毒DNA检测结果显示,宫颈炎、CINⅠ、CINⅡ～Ⅲ和宫颈癌患者各组HPVDNA检出率依次足2.5%、12.5%、68.0%、96.4%,EBV DNA为0、3.1%、28.0%和69.6%,其中宫颈炎组与CINⅡ～Ⅲ和宫颈癌组问的差异具有统计学意义(P均0.05),但宫颈炎与CINI组间的差异无统计学意义(P0.05)。宫颈病变病理进程不仅伴随着HPV及EBVDNA阳性检出率梯度上升,而且与HPV和EBV双重感染检出率增高呈正相关(r=0.46,X~2=82.50,P0.001)。对宫颈癌组织进行免疫组织化学分析,发现EBV DNA检测阳性标本中EBV蛋白阳性表达检出率为89.7%(34/39),而阴性标本中蛋白表达阳性率为6%(1/17)。结论:维吾尔族妇女宫颈癌的发生与发展可能与HPV和EBV双重感染密切相关。

The role of EBV and HPV infection in cervical cancer development of Uyghur women

OBJECTIVE:Study on the role of Ebstein-Barr virus(EBV) and human papillomavirus(HPV) infection in the development of cervical cancer of Uyghur women.METHODS:We collected 178 cases of formalin-fixed and paraffin-embedded cervical tissue specimens of Uyghur women with cervicitis,cervical intraepithelial neoplasia (CIN)Ⅰ/Ⅱ/Ⅲand cervical squamous cell carcinoma(CSCC).EBV and HPV DNA was detected by polymerase-chain reaction(PCR) with the tissue DNA extracted,and the EBV protein expression was checked by immunohistochemistry. RESULTS:HPV DNA was detectable in 2.5%,12.5%,68.0%and 96.4%cases of cervicitis,CINⅠ,CINⅡ-Ⅲand cervical cancer,respectively;and EBV DNA in 0%,3.1%,28.0%and 69.6%cases,respectivelly;with a significant difference among groups of cervicits,CINⅡ-Ⅲand cancer for both HPV and EBV(P>0.05).Further analysis indicated that cervical lesion pathogenesis was not only accompanied with gradual increasing rate for HPV or EBV DNA alone,but also positively correlated with the increase of HPV-EBV dual-infection(correlation coefficient r=0.46;x~2=82.50, P < 0.01).EBV protein expression was 89.7%positive in EBV-DNA positive cases(34/39)and 6%positive in EBV-DNA negative cases(1/17).CONCLUSION:Uyghur cervical cancer development and progression may be closely associated with the dual infection by HPV and EBV.