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| 结核分支杆菌早期分泌性蛋白ESAT-6基因真核表达重组质粒的构建及鉴定 | |
| 引用本文： | 蒋玉凤,张建军,钟森,史小玲,邓存良,王明勇. 结核分支杆菌早期分泌性蛋白ESAT-6基因真核表达重组质粒的构建及鉴定[J]. 泸州医学院学报, 2003, 26(6): 498-500 |
| 作者姓名： | 蒋玉凤 张建军 钟森 史小玲 邓存良 王明勇 |
| 作者单位： | 泸州医学院附属医院感染病科,四川泸州,646000 |
| 摘 要： | 目的 :构建结核分支杆菌早期分泌性蛋白ESAT - 6真核表达重组质粒。方法 :以结核杆菌H37Rv株基因组DNA为模板 ,用PCR法对ESAT - 6基因进行扩增 ,PCR反应产物经酶切后 ,克隆于真核表达载体pcDNA3.1( )上。结果 :构建了结核杆菌基因ESAT - 6真核表达重组质粒pcDNA3.1( ) -ESAT - 6 ,测序报告ESAT - 6基因已正向克隆到真核表达质粒pcDNA3.1( )上。结论 :结核杆菌早期分泌性蛋白ESAT - 6真核表达重组质粒的成功构建为结核病的诊断、重组疫苗应用和免疫效应检测打下了基础。 |
| 关 键 词： | 结核分枝杆菌 早期分泌性蛋白 克隆 真核表达 |
| 文章编号： | 1000-2669(2003)6-0498-03 |
| 修稿时间： | 2003-07-20 |
CONSTRUCTION AND IDENTIFICATION OF EUKARYOTIC EXPRESSION RECOMBINANT PLASMID OF ESAT-6 FROM MYCOBACTERIUM TUBERCULOSIS |
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| Jiang Yufeng,et al. CONSTRUCTION AND IDENTIFICATION OF EUKARYOTIC EXPRESSION RECOMBINANT PLASMID OF ESAT-6 FROM MYCOBACTERIUM TUBERCULOSIS[J]. Journal of Luzhou Medical College, 2003, 26(6): 498-500 | |
| Authors: | Jiang Yufeng et al |
| Affiliation: | Jiang Yufeng,et al Department of Infectious disease,Luzhou Medical hospital |
| Abstract: | Objective: To Construct eukaryotic expression recombinant plasmid of ESAT-6. Methods: The gene encoding for protein ESAT-6 was amplified from M.tuberculosis H37Rv genomic DNA by using PCR technique and ESAT-6 gene was ligated to eukaryotic expression vector pcDNA3.1( ).Result: ESAT-6 gene was successfully inserted into the eukaryotic expression vector pcDNA3.1( ).Conclusion: The recombinant eukaryotic expression plasmid pcDNA3.1( )-ESAT-6 has been constructed. It may lay foudations for the diagnosis, the application of new vaccine of tuberculosis and the detecting of immunity effectiveness. |
| Keywords: | Mycobacterium tuberculosis ESAT-6 Eukaryotic expression |
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