| 细胞增殖法检测单核/巨噬细胞ADCC效应方法的建立 |
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| 引用本文： | 黄瑾,马忠森,林树青,王荣有. 细胞增殖法检测单核/巨噬细胞ADCC效应方法的建立[J]. 中国免疫学杂志, 1999, 0(4): 177-179 |
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| 作者姓名： | 黄瑾 马忠森 林树青 王荣有 |
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| 作者单位： | 1. 白求恩医科大学第二临床学院呼吸内科,长春,130041
2. 白求恩医科大学第二临床学院胸外科,长春,130041 |
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| 摘 要： | 目的：为了建立一种供临床实验室广泛应用的检测单核／巨噬细胞ＡＤＣＣ效应的实验方法。方法：选择ＣＴＬＩ２细胞作为靶细胞，制备出兔抗ＣＴＬＩ２细胞特异性抗血清，用单核细胞作为效应细胞，通过ＣＴＬＩ２细胞增殖结果检测单核细胞ＡＤＣＣ效应，并与传统检测方法＾１２５Ｉ－ＵｄＲ释放试验进行比较。结果：该方法较同位素试验方法更敏感。结论：细胞增殖法检测单核细胞ＡＤＣＣ效应准确、可靠。
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| 关 键 词： | 巨噬细胞 ADCC 细胞增殖 检测 单核细胞 |
Creation of the method of cell proliferation for detecting the ADCC effect of monocyte/macrophage |
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| HUANG Jin,MA Zhong Sen,LIN Shu Qing et al. Creation of the method of cell proliferation for detecting the ADCC effect of monocyte/macrophage[J]. Chinese Journal of Immunology, 1999, 0(4): 177-179 |
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| Authors: | HUANG Jin MA Zhong Sen LIN Shu Qing et al |
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| Affiliation: | HUANG Jin,MA Zhong Sen,LIN Shu Qing et al. The Second Clinical College of Norman Bethune University of Medical Sciences,Changchun 130041 |
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| Abstract: | Objective:To create a method of detecting the ADCC effect of monocyte/macrophage in clinical laboratory.Methods:CTLL2 cells was selected as target cells to prepare the specific antiserum of rabbit.Monocytes act as effect cells.The ADCC effect of monocyte was detected by observing the proliferation of CTLL2 cells.This new method was also compared with the traditional method of 125 I UdR releasing test.Results:The method of cell proliferation is more sensitive than that of isotope releasing test.Conclusion:Using cell proliferation method to detect the ADCC effect of monocyte is stable and accurate. |
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| Keywords: | Monocyte/macrophage ADCC Cell proliferation |
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