| Growth differentiation factor-5 stimulates the growth and anabolic metabolism of articular chondrocytes |
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| 引用本文： | Xu Peng Guo Xiong Yao Jianfeng Zhang Yingang Klaus von der Mark. Growth differentiation factor-5 stimulates the growth and anabolic metabolism of articular chondrocytes[J]. 美中国际创伤杂志, 2005, 4(4): 1-6 |
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| 作者姓名： | Xu Peng Guo Xiong Yao Jianfeng Zhang Yingang Klaus von der Mark |
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| 作者单位： | [1]Department of Bone and Joint Diseases, Xi＇an Red Cross Hospital, Xi＇an 710054,China [2]Institute of Endemic Diseases, Medical School of Xi＇an Jiaotong University, Xi＇an 710061, China [3]Department of Experimental Medicine I, University of Erlangen-Nuernberg, Erlangen 91054, Germany |
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| 摘 要： | Objective： To observe the effect of growth differentiation factor-5 （GDF-5） on the growth and anabolic metabolism of articular chondrocytes. Methods： The articular chondrocytes isolated from rats were treated with various concentrations of rmGDF-5, and the growth of chondrocytes measured by MTT assay, the cellular cartilage matrices formation detected sulfated glycosaminoglycan by Alcian blue staining and type Ⅱ collagen by RT-PCR, the collagen phenotypic expression of chondrocytes detected by immunofluorescence. Results： After 7 days culture, MTT assay showed that GDF-5 enhanced the growth of chondrocytes in a dose-dependent manner, RT-PCR showed that GDF-5 clearly induced the synthesis of type Ⅱcollagen because of the col2al mRNA band more and more strong in a dose-dependent. Chondrocytes were cultured with GDF-5 for 14 days, the intensity of Alcian blue staining was greatly enhanced, especially, at a high concentration of 1000ng/ml, and GDF-5 enhanced the accumulation of the Alcian blue-stainable material in a concentration-dependent manner and in a does-dependent manner. Chondrocytes were cultured with GDF-5 for 21 days, immunofluorescent staining of type Ⅱ collagen was clear, the type Ⅰ and Ⅹ collagen were negative. Conclusion： GDF-5 enhanced the growth of mature articular chondrocytes, and stimulated the cellular cartilage matrices formation, but did not change the collagen phenotypic expression of chondrocytes in mono-layer culture.
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| 关 键 词： | 生长分化因子-5 合成代谢 关节 软骨细胞 |
| 文章编号： | 1538-814X（2005）04-0001-06 |
Growth differentiation factor-5 stimulates the growth and anabolic metabolism of articular chondrocytes |
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| Xu;Peng;Guo;Xiong;Yao;Jianfeng;Zhang;Yingang;Klaus;von;der;Mark. Growth differentiation factor-5 stimulates the growth and anabolic metabolism of articular chondrocytes[J]. U.S.Chinese International Journal of Traumatology, 2005, 4(4): 1-6 |
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| Authors: | Xu Peng Guo Xiong Yao Jianfeng Zhang Yingang Klaus von der Mark |
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| Abstract: | |
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| Keywords: | Growth differentiation factor-5 Articular ehondroeytes Cell growth Matrix formation Collagen pheno typicexpression Rat |
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