Normal values at 37 degrees for serum lactate dehydrogenase, alpha-hydroxybutyrate dehydrogenase and glycerate dehydrogenase

The distribution of serum lactate dehydrogenase and α-hydroxybutyrate dehydrogenase activity in a sample population of 280 volunteers is presented. Enzyme activity was measured at 37° using optimal concentrations of substrate, coenzyme and phosphate buffer. Significant increases in lactate dehydrogenase activity using the substrates pyruvate, 2-oxobutyrate and hydroxypyruvate were found with increasing age. No significant differences between males and females were found with pyruvate as substrate, but were found when 2-oxobutyrate and hydroxypyruvate were used.