周围神经损伤后选择性再生的实验动物模型研究

目的:建立并评价周围神经损伤后神经选择性再生的实验动物模型。方法:3周龄SD大鼠12只随机分成4周组和8周组各6只,均行切断右侧L2～L5背根神经节后纤维和右侧股神经横切-吻合手术,分别于术后4周和8周进行荧光逆向示踪,观察脊髓运动神经元再生情况。结果:4周组大鼠长入运动神经、感觉神经和长入2种神经的运动神经元数量分别为(167±21)个、(53±14)个、(36±7)个;8周组分别为(252±14)个、(37±8)个、(21±4)个。结论:该实验动物模型能反映周围神经损伤后运动神经再生的倾向性,重复性好。

Establishment of a Modified Selective Regeneration Model on Rats after Peripheral Nerve Injury

Objective:To establish a rat modified selective regeneration model after peripheral nerve injury. Methods: Twelve 3-week-old SD rats were randomly divided into 4-week group (n=6) and 8-week group (n=6). The postganglionic fibers of L2~L5 dorsal root ganglia on the right side were cut off and the right femoral nerve was transectioned and then sutured in all rats. At the 4th week (4-week group) or 8th week (8-week group) after operation,the retrograde tracers (fluorescein dextran and tetramethylrhodamine dextran) were used to investigate the regeneration of the motor neurons in the spinal cord after injury. Results: In 4-week group,the numbers of neurons regenerated into the motor and sensory nerves and the both were (167±21),(53±14) and (36±7) respectively. In 8-week group the numbers were (252±14),(37±8) and (21±4) respectively. Conclusion: It is an ideal rat model with good repeatability to study selective regeneration after peripheral nerve injury.