Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis |
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Authors: | Nagata Reiko Muneta Yoshihiro Yoshihara Kazuhiro Yokomizo Yuichi Mori Yasuyuki |
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Affiliation: | Immune System Section, Department of Immunology, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan 305-0856. kikuma@affrc.go.jp |
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Abstract: | Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family. |
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