| 光照应激对SD大鼠小肠中annexin 5表达的影响 |
| |
| 引用本文： | 时姗姗,卢坤刚,柳海燕,陶晓倩,林钗英,姚兵. 光照应激对SD大鼠小肠中annexin 5表达的影响[J]. 临床检验杂志, 2010, 28(3): 221-223 |
| |
| 作者姓名： | 时姗姗 卢坤刚 柳海燕 陶晓倩 林钗英 姚兵 |
| |
| 作者单位： | 南京师范大学生命科学院;南京军区南京总医院解放军临床检验医学研究所; |
| |
| 基金项目： | 国家自然科学基金(30770801);;全军“十一五”医药卫生科研基金资助项目(06H011) |
| |
| 摘 要： | 目的观察光照应激状态下SD大鼠小肠黏膜组织中annexin 5的分布和表达变化。方法建立SD大鼠光照应激模型,24 h持续光照,分为短期光照应激(2、3、4、7 d)和长期光照应激(14、21、28 d),分别取应激组和相应对照组的大鼠小肠组织,采用免疫组织化学和western blot技术分析annexin 5在各时间段大鼠小肠组织中的分布和表达。结果western blot结果显示,与对照组相比,持续光照2、3、4、7 d,实验组大鼠小肠中annexin 5表达无明显变化;持续光照14 d和21 d,实验组大鼠小肠中annexin 5的表达量显著高于对照大鼠,具有统计学意义(P<0.05);持续光照28 d,实验组大鼠小肠中annexin 5表达量虽仍高于对照鼠,但无显著性差异(P>0.05)。免疫组织化学显示,annexin 5在大鼠小肠中的分布未见明显改变,但光照应激后期实验组(14、21、28 d)annexin 5免疫组化显色较深。结论在光照应激后期(14、21 d)annexin 5的表达量明显增加。随着应激时间的延长(28 d),机体内代偿机制的建立,实验组annexin 5逐渐恢复至正常水平...
|
| 关 键 词： | 光照 应激 小肠 annexin5 |
| 收稿时间： | 2009-12-20 |
Effect of constant light stress on annexin 5 in small intestine of Sprague-Dawley rats |
| |
| SHI Shan-shan,LU Kun-gang,LIU Hai-yan,TAO Xiao-qian,LIN Chai-ying,YAO Bing. Effect of constant light stress on annexin 5 in small intestine of Sprague-Dawley rats[J]. Chinese Journal of Clinical Laboratory Science, 2010, 28(3): 221-223 |
| |
| Authors: | SHI Shan-shan LU Kun-gang LIU Hai-yan TAO Xiao-qian LIN Chai-ying YAO Bing |
| |
| Affiliation: | 1.College of Life Science/a>;Nanjing Normal University/a>;Nanjing 210097/a>;2.Institute of Clinical Laboratory Medicine of PLA/a>;Nanjing General Hospital of Nanjing Command/a>;Nanjing 210002/a>;China |
| |
| Abstract: | Objective To observe the expression of annexin 5 in the small intestine of Sprague-Dawley(SD) rats with constant-light stress.Methods SD rats were continuously exposed to light to establish short(2,3,4,and 7 d) and chronic(14,21,and 28 d) constant-light stressed models.Small intestines were taken from the light exposed and unexposed rats respectively.Immunoreactivity and Western blotting were used to observe the localization and expression of annexin 5 in rat small intestines respectively.Results Western bl... |
| |
| Keywords: | constant light stress small intestine annexin 5 |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《临床检验杂志》浏览原始摘要信息 |
|
点击此处可从《临床检验杂志》下载全文 |
|
