重组腺病毒介导TIMP-1对人肝癌细胞系体外侵袭的影响

[目的]探讨人基质金属蛋白酶组织抑制因子-1(hTIMP-1)过表达对人肝癌细胞系HepG2体外侵袭的作用。[方法]构建携载hTIMP-1全长cDNA的重组腺病毒AdhTIMP-1,转染HepG2细胞,利用MTT、细胞生长曲线及BoydenChamber检测hTIMP-1对HepG2细胞体外增殖和侵袭能力的影响。[结果]成功构建重组腺病毒并实现体外表达,转染HepG2细胞,对其体外增殖及侵袭有明显抑制作用,细胞增殖率为49%,穿膜细胞相对百分率为(8.4±1.2)%(P<0.01)。[结论]hTIMP-1的过表达能有效抑制人肝癌细胞系HepG2的体外侵袭,可望用于肝癌基因治疗的研究。

Effects of recombinant adenovirus-mediated overexpression of TIMP-1 on invasion of human liver carcinoma cell line in vitro

[Objective] To explore the effects of overexpression of human tissue inhibitors of metalloproteinase-1 (hTIMP-1) on invasion of human liver carcinoma cell line HepG2 in vitro. [Methods] A recombinant adenoviral vector containing full-length cDNA of hTIMP-1 was generated by homologous recombination and was transfected into HepG2. MTT, growth curve, and Boyden Chamber were undertaken to assay the change of proliferation and invasion of transfected cell lines in vitro. [Results] The resultant AdhTIMP-1 was successfully constructed and the expression of hTIMP-1 was detected in vitro. Having been transfected with AdhTIMP-1, the proliferation and invasion of HepG2 were significantly inhibited. The suppression rate of HepG-2 cells with AdhTIMP-1 transfection was 49.5% and AdhTIMP-1 transfection inhibited by more than 91.6% the invasion into the Matrigel-coated filter (P<0.01). [Conclusion] The invasion of human liver carcinoma cell line was markedly inhibited by recombinant adenovirus-mediated overexpression of hTIMP-1 and may paved the way for further application in liver gene therapy.