尿毒症患者血清对大鼠系膜细胞增殖的影响

取尿毒症患者血清,稀释成不同浓度,体外培养大鼠系膜细胞(MC),采用四甲基偶氮唑蓝(MTT)法检测细胞增殖,流式细胞仪测定细胞周期,免疫细胞化学法检测C-jun蛋白表达.发现不同稀释浓度尿毒症患者血清抑制大鼠MC增殖,具有浓度和时间依赖性;并促使S+G2/M期细胞减少,G0～G1期细胞增多;明显抑制C-jun蛋白的表达(P<0.01,P<0.05).同时,高甲状旁腺激素(PTH)尿毒症患者血清的抑制作用大于PTH正常的患者血清(P<0.05).提示慢性肾衰竭晚期尤其合并甲状旁腺功能亢进时,MC增殖已不是导致病程进展的主要原因.

Effect of uremia serum on proliferation of rat mesangial cells cultured in vitro

Serum of uremia patients was diluted into different concentrations and was co cultured with rat mesangial cells(MC).Cell proliferation was assessed by MTT colorimetric assay;cell cycle was analyzed by flow cytometry;the expression of C jun protein was determined by immunocytochemistry. It was found that serum of uremia patients with different dilution inhibited the proliferation of rat MC in a time and dose dependent manner,increased the G 0 G 1 phase cells, decreased the S+G 2/M phase cells,and markedly inhibited expression of C jun protein( P <0.01, P <0.05).The inhibition effect of uremia serum with high PTH was stronger than that with normal PTH( P <0.05).The results suggested that at the late stage of chronic renal failure(CRF),especially when complicated with hyperparathyroidism,the proliferation of MC is not the major reason for CRF progress.