RNA干扰抑制视网膜母细胞瘤细胞血管内皮生长因子的表达

目的利用RNA干扰（RNAi）技术,研究血管内皮生长因子（VEGF）编码基因的siRNA对人视网膜母细胞瘤（RB）细胞的影响,从而探讨通过抑制血管内皮生长因子在视网膜母细胞瘤组织中的表达来治疗视网膜母细胞瘤的机理。方法将VEGF编码基因特异的siRNA转染入视网膜母细胞瘤细胞,采用RT PCR和Western blot技术检测siRNA处理前后RB细胞VEGF基因表达变化。MTT法检测siRNA处理前后RB细胞生长的抑制情况。结果RT PCR和Western blot均显示VEGF特异性siRNA对视网膜母细胞瘤细胞血管内皮生长因子基因的表达有抑制作用,MTT法检测RB细胞生长的抑制情况显示,抑制率可达40%。结论VEGF特异性siRNA的特异性基因沉默作用对RB细胞VEGF基因的表达有抑制作用。

RNA interference inhibits VEGF expression in retinoblastoma cells: an experimental study

Objective To explore the possibility of VEGF as a therapeutic target for retinoblastoma,we employed RNA interference to inhibit VEGF expression in retinoblastoma cells.Methods SiRNA oligo of VEGF was synthesized and transfected into retinoblastoma cells.VEGF mRNA expression and protein content were respectively determined by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.Retinoblastoma cell proliferation was detected by MTT reduction assay.Results VEGF mRNA expression and protein c...