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| HPLC测定淡竹叶中日本当药黄素的含量 | |
| 引用本文： | 张靖,王春华,王勇,王英,李药兰,叶文才. HPLC测定淡竹叶中日本当药黄素的含量[J]. 中国实验方剂学杂志, 2011, 17(6): 51-53 |
| 作者姓名： | 张靖 王春华 王勇 王英 李药兰 叶文才 |
| 作者单位： | 1. 暨南大学中药及天然药物研究所,广州,510632;广东省中医院中药制剂实验室,广州,510120 2. 暨南大学中药及天然药物研究所,广州,510632;中药药效物质基础及创新药物研究广东省高校重点实验室,广州,510632 3. 江苏省食品药品检验所,南京,210008 |
| 基金项目： | 广东省自然科学基金团队项目，国家自然科学基金面上项目，澳门特别行政区科学技术发展基金 |
| 摘 要： | 目的:建立中药淡竹叶中日本当药黄素的RP-HPLC含量测定方法。方法:高效液相色谱法,采用Cosmosil 5 C18-ms-Ⅱ色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.05%醋酸水溶液(14:86),流速1.0 mL·min-1,分析时间20 min,检测波长350 nm,进样体积10μL。结果:日本当药黄素进样量与峰面积呈良好线性关系,线性范围为0.3～5.0μg,r=1.000 0(n=5),回收率为100.2%(n=6)。结论:该方法首次测定了8个地区淡竹叶药材中日本当药黄素的含量,该方法准确、简便、重复性好。 |
| 关 键 词： | 淡竹叶 日本当药黄素 高效液相色谱 含量测定 |
| 收稿时间： | 2010-10-11 |
HPLC Determination of Swertiajaponin in Leaves of Lophatherum gracile 1,3* |
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| ZHANG Jing,WANG Chun-hu,WANG Yong,WANG Ying,LI Yao-lan and YE Wen-cai. HPLC Determination of Swertiajaponin in Leaves of Lophatherum gracile 1,3*[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(6): 51-53 | |
| Authors: | ZHANG Jing WANG Chun-hu WANG Yong WANG Ying LI Yao-lan YE Wen-cai |
| Affiliation: | Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China; Preparation Laboratory of Traditional Chinese Medicine,Guangdong Hospital of Traditional Chinese Medicine,Guangzhou 510120,China;Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Tratitional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China;Jiangsu Institute for Food and Drug Control, Nanjing 210008, China;Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Tratitional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China;Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Tratitional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China;Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Tratitional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China |
| Abstract: | |
| Keywords: | Lophatherum gracile swertiajaponin HPLC content determination |
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