抗柯萨奇B病毒性心肌炎胶囊复方水提物的 镇痛和抗应激作用

目的:观察抗柯萨奇B病毒性心肌炎胶囊(K-CoxB-JN)复方水提物镇痛作用和抗应激反应能力。方法:健康昆明种小鼠随机分为5组,K-CoxB-JN复方水提物高剂量组、中剂量组、低剂量组,20 mL·kg-1(给药量为9.6,4.8,2.4 g·kg-1),分别连续ig给药,并设阳性对照组及正常对照组。采用醋酸和热刺激致疼痛模型,考察其镇痛活性;采用游泳实验和耐缺氧实验,考察其抗应激作用。结果:K-CoxB-JN复方水提物对小鼠抗疲劳、耐缺氧等抗应激能力有一定的增强作用。相对于对照组,高剂量给药组可使小鼠游泳时间延长57 s,耐缺氧存活时间延长12 min;可以明显抑制醋酸引起的扭体次数,延长热板上小鼠舔后足出现的时间。相对于对照组,高剂量给药组可使小鼠扭体次数减少20次;中剂量给药组可使小鼠舔后足时间延长16s。结论:K-CoxB-JN复方水提物具有明显的镇痛和抗应激作用。

Experimental Studies of Water Extraction of K-CoxB-JN on Anti-stress and Analgesic Activities

Objective: To study the anti-stress and analgesic activities of water extraction of K-CoxB-JN, a compound Chinese medicine for treatment of myocarditis. Method: Water extraction of K-CoxB-JN was consecutively given to healthy Kunming mice with high (9.6 g ·kg^-1), medium (4.8 g ·kg^-1) and low dose (2.4 g ·kg^-1) ig. At the same time, control group and positive group were set up. Analgesic activity was evaluated in mice model of pain induced by thermal stimulus and acetic acid. Meanwhile, anti-stress activity was evaluated by swimming and anti-hypoxia experiments. Result: Water extraction of K-CoxB-JN could enhance anti-stress capability of anti-fatigue and anti-hypoxia. The number of writhing induced by acetic acid was significantly reduced and the time of licking rear feet induced by hot plate stimulation was prolonged. Conclusion: Water extraction of K-CoxB-JN has good effects of anti-stress and analgesic capability.