脱细胞尿道及其海绵体基质制备的实验研究

目的 探索脱细胞尿道及其海绵体基质的制备方法。方法取健康壮年兔完整尿道及其海绵体组织，以Triton-X100与NH3H2O联合提取法进行脱细胞处理。标本做HE染色，组织学观察分析脱细胞效果。结果脱细胞处理11天后，成功获得脱细胞及其海绵体基质，所得基质外观良好。HE染色观察无细胞存在。弹力纤维排列规整，间隙较大，结构无破坏。结论利用Triton-X100与NH3H2O联合提取法可成功制备完整无细胞尿道及其海绵体基质，为尿道再造修复提供崭新思路。

Preparing acellular urethral and cavernous tissue matrix

Objective To explore the method of preparing acellular urethral and its cavernous tissue matrix. Methods The whole urethral and its cavernous tissue were harvested from healthy rabbit and then the cells were removed from the tissue with Triton - X 100 and NH3H2O. Specimens were stained with haematoxylin - eosin and observed under the light microscopy. Results The acellular urethral and cavernous tissue matrix was harvested successfully after that the urethral tissue had been processed with Triton- X 100 and NH3H2O for 11 days. The matrix looks satisfied. Light microscopy revealed that there's no cells in the ACTM, furthermore the elastin fibers arranged regularly and the frame was not destroyed, but the space was bigger than that before. Conclusion Acellular urethral and cavernous tissue matrix can be obtained successfully through the method of using Triton - X 100 association with NH3H2O. This procedure give us a new thought to repair the urethral diseases.