同种异体移植子宫内膜异位症大鼠模型的建立

目的建立Wistar大鼠同种异体子宫内膜异位症模型。方法采用外科诱导法对24只Wistar雌性大鼠行异体子宫组织移植手术,30只行自体子宫组织移植手术。结果 Wistar大鼠造模12周后,异体移植成模率83.3%,与自体移植成模率76.7%比较差异有统计学意义（P〈0.05）;异体、自体模型异位内膜上皮细胞功能活跃,有类似正位子宫内膜的周期变化和生理特征;且造模后大鼠体液免疫反应敏感性与假手术对照组比较,C3、C4、IgG升高,IgM降低,差异有统计学意义,IgA没有明显变化;异体移植模型IgG比自体移植模型高,差异有统计学意义。结论应用Wistar大鼠进行同种异体移植手术可以成功的建立子宫内膜异位症模型。

Establishment of An Allograft Rat Model for the Study of Endmetriosis

Objective To establish an allograft endmetriosis model.Methods 24 female Wistar rats were used to induce endometriosis through allogeneic uterus transplants.30 female Wistar rats received autologous uterus transplant were used as control.Results 12 weeks after transplantation,the successful rate with allograft method in rats was 83.3%,and that the autologous was 76.7%,and there was significant difference.The activities of epithelial cells of endometria in both autologous and allogenic transplantation groups were similar to the activities of epithelial cells in the normal endometria.And they had the similar period changes and physiological characteristics to the entopic endometrium.The humoral response and the level of C3,C4 and IgG were also elevated in the rats receiving allogenic transplantation.However,the level of IgM was lower and the level of IgA was not changed.The concentration of IgG for the allogeneic tissues was significantly higher than the rats receiving autologous transplantation.Conclusion A model of endometriosis by allograft transplantation was successfully established using Wistar rat.