| 流感病毒MDCK细胞培养条件的优化 |
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| 引用本文： | 莫浩联,司徒潮满,侯红斌,方良,陈润莉,张勇. 流感病毒MDCK细胞培养条件的优化[J]. 广东寄生虫学会年报, 2010, 0(9): 1076-1077 |
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| 作者姓名： | 莫浩联 司徒潮满 侯红斌 方良 陈润莉 张勇 |
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| 作者单位： | 深圳市福田区疾病预防控制中心,深圳518040 |
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| 摘 要： | 目的优化流感病毒MDCK细胞培养条件,提高流感病毒分离率和流感监测效果。方法使用MDCK细胞培养法,比较传统的与优化的实验条件对流感病毒分离率和对血凝试验滴度的影响。结果不同的优化实验条件可明显提高流感病毒的分离率和滴度。结论已筛选出流感病毒MDCK细胞培养法适宜的条件。
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| 关 键 词： | 细胞培养 流感病毒 病毒分离 |
Optimization of MDCK Cell Culturing Condition for the Cultivation of Influenza Virus |
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| MO Hao-lian,SITU Chao-man,HOU Hong-bin,FANG Liang,CHEN Run-li,ZHANG Yong. Optimization of MDCK Cell Culturing Condition for the Cultivation of Influenza Virus[J]. Journal of Tropical Medicine, 2010, 0(9): 1076-1077 |
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| Authors: | MO Hao-lian SITU Chao-man HOU Hong-bin FANG Liang CHEN Run-li ZHANG Yong |
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| Affiliation: | (Shenzhen Futian Center for Disease Control and Prevention, Shenzhen 518040, China) |
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| Abstract: | Objective To optimize the cultivation conditions for MDCK cells for the isolation and cultivation of influenza virus.Methods Compare the rate of influenza virus isolation and the titer of hemagglutination before and after the optimization of the MDCK culturing conditions.Results The optimized culture conditions can significantly improve the isolation rate of influenza viruses and the results of virus titration.Conclusion We have optimized the conditions for the cultivation of MDCK cells. |
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| Keywords: | cell culture influenza virus virus isolation |
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