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| FOLIN-酚比色法测定大风藤中总酚含量的研究 | |
| 引用本文： | 吴蓓,邓志清,余姝,冯育林,刘受先,杨世林.FOLIN-酚比色法测定大风藤中总酚含量的研究[J].江西中医学院学报,2011,23(1):43-45. |
| 作者姓名： | 吴蓓 邓志清 余姝 冯育林 刘受先 杨世林 |
| 作者单位： | 1. 江西中医学院,南昌,330006;江西省南昌市食品药品检验所,南昌,330038 2. 江西中医学院,南昌,330006;中药固体制剂制造技术国家工程研究中心,南昌,330006 3. 江西中医学院,南昌,330006 |
| 摘 要： | 目的:建立大风藤总酚的测定方法。方法:采用Folin-酚比色法,以白藜芦醇为对照品分析测定了大风藤中总酚含量。结果:大风藤提取液在加入Folin-酚试3 ml、7.5%Na2CO33 ml,反应温度为50℃,反应时间为90 min,浓度在0.672 mg/ml~4.704 mg/ml时与其吸光度值呈良好的线性关系,其回归方程A=1.509 9C+0.051 9(n=7),相关系数为r=0.999 15,根据此线性回归方程进行大风藤总酚的定量测定。结论该方法简单方便、稳定性好、准确度高。 |
| 关 键 词： | 大风藤 总酚 Folin-酚 |
Study on Determination of Total Hydroxybenzene in Parthenocissus tricuspidata(Sieb et Zucc)Planch.by Folin-phonel Colorimetry |
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| WU Bei,DENG Zhi-qing,YU Shu,FENG Yu-lin,LIU Shou-xian,YANG Shi-lin.Study on Determination of Total Hydroxybenzene in Parthenocissus tricuspidata(Sieb et Zucc)Planch.by Folin-phonel Colorimetry[J].Journal of Jiangxi College of Traditional Chinese Medicine,2011,23(1):43-45. | |
| Authors: | WU Bei DENG Zhi-qing YU Shu FENG Yu-lin LIU Shou-xian YANG Shi-lin |
| Institution: | 1,21.Jiangxi Universty Of Traditional Chinese Medicine,Nanchang 330006,China 2.National Pharmaceutical Engineering Center For Solid Preparation in Chinese Herbal Medicine,Nanchang 330006,China; 3.Nanchang Institute for Drug Control;Nanchang330038,China |
| Abstract: | Objective：To establish a method for determination of total hydroxybenzene in Parthenocissus tricuspidata（Sieb et Zucc） Planch..Methods：With resveratrol as the standard,the content of total hydroxybenzene in Parthenocissus tricuspidata（Sieb et Zucc） Planch.was determined by Folin-phonel colorimetry.Results：The polyphenol content of Parthenocissus tricuspidata（Sieb et Zucc） Planch.could be well calculated by applying Folin-Ciocalteu reagent 3 ml,7.5% Na2CO3 3ml,90min at 50℃ for colorimetric reaction,and the linear range of standard curve was 0.672mg/ml～4.704mg/ml.The regression equation was found as follow： A=1.5099C＋0.0519（n=7） with the correlation coefficent r=0.999 15.Conclusion The method established was very conwenient,stable and accurate. |
| Keywords: | Parthenocissus tricuspidata（Sieb et Zucc）Planch Total hydroxybenzene Folin-phonel |
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