BACTEC 9050自动血培养仪的临床应用

目的 评价BACTEC 9050自动血培养仪的临床应用情况。方法 分析2年来自动血培养仪检测的阳性率、阳性报警时间、菌种鉴定及临床分析。结果 检测2424份血培养标本，分离出328株菌，总阳性率为13．5％（328／2424），其中革兰阴性菌167株（50．91％，167／328），阳性菌112株（34、15％，112／328），真菌49株（14．94％，49／328）。分离率居前三位的细菌是大肠埃希菌（22．86％），金黄色葡萄球菌和肺炎克雷白菌（10．36％），白色念珠菌（7、32％）。在全部328例阳性瓶中，24h阳性报告率为51．36％，48及72h阳性率分别为78．5％、92．3％。结论 BACTEC 9050自动血培养仪对肠杆菌、葡萄球菌及真菌具有较好的检测能力。

Clinical Application and Evaluation of BACTEC 9050 Automated Blood Culture System

Objective To evaluate the clinical application of BACTEC 9050 automated blood culture system.Methods Two thousand four hundred and twenty-four blood samples were detected by BACTEC 9050 automated blood culture system.The types of microorganisms recovered and the positive rate were also analyzed.Results Three hundred and thirty-one samples were positive with alarm and 328 strains were isolated.Positive rate was 13.5%,of which,167((50.91%)) were gram negative bacteria,112(34.15%) were gram negative cocci,49(14.94%) were fungus.The most common pathogens were E.coli,Staphylococcus aureaus,K.pneumoniae and Candida albicans.The cumulative positive rate was 51.36% within 24h,78.5% within 48 h and 92.3% within 72 h.Conclusion BACTEC 9050 automated blood culture system is applicable in clinical use especially for aerogens,Staphylococcus and fungus.