银杏内酯B对牛主动脉平滑肌细胞增殖的影响

目的探讨银杏内酯B(GB)对牛主动脉平滑肌细胞(SMC)增殖的影响及可能机制。方法细胞的增殖由³H-胸腺嘧啶核苷掺入试验确定,应用流式细胞术测定细胞周期,用MTT法和乳酸脱氢酶活性检测观察细胞代谢的变化。结果无论是否用血管紧张素II诱导,GB与银杏内酯A和B的混合物均呈浓度依赖性地抑制SMC的增殖,其机制与阻止细胞进入有丝分裂期有关。结论GB可抑制动脉壁SMC的增殖,该作用不完全取决于血小板活化因子受体的拮抗活性。

INHIBITORY EFFECTS OF GINKGOLIDE B ON PROLIFERATION OF BOVINE AORTIC SMOOTH MUSCLE CELLS

AIM: To investigate the effects of ginkgolide B (GB) on proliferation of bovine aortic smooth muscle cells (SMC) and its related mechanisms. METHODS: After pretreating with GB or the mixture of ginkgolide A and B (GA:GB) at 37 degrees C for 0.5 h, the VSMC were treated with or without angiotensin II (Ang II) for 24 h. The proliferation of SMC was evaluated by 3H-thymidine incorporation and the cell cycle phase was measured by flow cytometry. Cytotoxicity was reflected by MTT and lactate dehydrogenase (LDH) activity of the supernatant. RESULTS: Whether or not treated with Ang II, GB and GA:GB were shown to suppress SMC proliferation in concentration-dependent fashion at concentrations ranging from 10(-9) mol.L-1 to 10(-5) mol.L-1. The inhibitory effects appeared to be related to a G1-->S block in cell cycle traverse. CONCLUSION: The suppression of SMC proliferation by GB might not only be contributed by blockage of the PAF receptor activity.