Identification of lymphogranuloma venereum‐associated Chlamydia trachomatis serovars by fluorescence in situ hybridisation – a proof‐of‐principle analysis |
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| Authors: | Hagen Frickmann Andreas Essig Sven Poppert |
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| Affiliation: | 1. Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, , Hamburg, Germany;2. Institute for Microbiology, Virology and Hygiene, University Hospital Rostock, , Rostock, Germany;3. Institute for Medical Microbiology and Hygiene, University Hospital Ulm, , Ulm, Germany;4. Institute for Medical Microbiology, Justus Liebig University Giessen, , Giessen, Germany;5. Bernhard Nocht Institute for Tropical Medicine, , Hamburg, Germany |
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| Abstract: | We describe a proof‐of‐principle evaluation of a fluorescence in situ hybridisation (FISH) procedure to identify Chlamydia trachomatis serovars L1–L3, the causative agents of lymphogranuloma venereum, in cell cultures based on newly designed DNA probes. Rapid and easy‐to‐perform FISH could facilitate the diagnosis of lymphogranuloma venereum without nucleic acid amplification or serotyping, but requires broader evaluation studies, for example, in tropical high‐endemicity regions. |
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| Keywords: | fluorescence in situ hybridisation lymphogranuloma venereum
Chlamydia trachomatis
cell culture diagnostic microbiology |
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