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Expression of Protease Activated Receptor‐1 in Chronic Periodontitis
Authors:Henrique Aparecido Bueno da Silva  Vanessa Tubero Euzebio Alves  Luis Carlos Spolidório  João Batista César Neto  Rosangela Santos Eichler  Maria Helena Catelli de Carvalho  Marinella Holzhausen
Affiliation:1. Division of Periodontics, Department of Stomatology, School of Dentistry, University of S?o Paulo, S?o Paulo, S?o Paulo, Brazil.;2. Department of Physiology and Pathology, Dental School at Araraquara, State University of S?o Paulo, Araraquara, S?o Paulo, Brazil.;3. Department of Pharmacology, Institute of Biomedical Sciences, University of S?o Paulo.
Abstract:Background: Protease activated receptor‐1 (PAR1) activation by thrombin may play a role in repair and homeostasis of periodontal tissues. The main objective of this study is to investigate PAR1 expression in patients with periodontitis, before and after non‐surgical periodontal treatment, and to associate its expression with the presence of inflammatory biomarkers and PAR2 expression. Methods: Gingival crevicular fluid (GCF) samples and clinical parameters, including probing depth, clinical attachment level, bleeding on probing, and gingival and plaque indices, were collected from periodontally healthy individuals and patients with moderate chronic periodontitis (CP) before and 6 weeks after periodontal non‐surgical treatment. PAR1 and PAR2 messenger RNA (mRNA) at the GCF were evaluated by quantitative polymerase chain reaction (qPCR). Flow cytometry analysis identified the GCF PAR1‐expressing cells. GCF inflammatory biomarkers were also determined. Results: Clinical parameters were significantly improved after therapy (P <0.01). The qPCR analysis showed that, before therapy, PAR1 mRNA levels in CP were similar to controls. Periodontal treatment led to increased PAR1 expression in CP (P <0.05). PAR1 expression was inversely correlated to PAR2 expression and with interleukins 6 and 8, tumor necrosis factor‐α, interferon‐γ, and matrix metalloproteinase‐2 levels. Conclusions: Periodontal treatment results in PAR1 overexpression in the GCF, and PAR1 expression is associated with decreased expression of inflammatory biomarkers and inversely correlated to PAR2 expression in the GCF. Therefore, the data suggest the importance of PAR1 mediating the known anabolic actions of thrombin in the periodontium.
Keywords:Biological markers  chronic periodontitis  gene expression  immunity, innate  inflammation  wound healing
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