| 重组腺病毒载体介导IL-12基因在间充质干细胞中的表达 |
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| 引用本文： | 王艳俊,隗玉川,杨智翔,应朝晖. 重组腺病毒载体介导IL-12基因在间充质干细胞中的表达[J]. 中国误诊学杂志, 2011, 11(3): 516-518 |
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| 作者姓名： | 王艳俊 隗玉川 杨智翔 应朝晖 |
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| 作者单位： | 大连大学附属新华医院胸外科,辽宁,大连,116021 |
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| 基金项目： | 大连市卫生局基金(编号:WSJ/KZT-01-ZL) |
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| 摘 要： | 目的将大鼠骨髓间充质干细胞(MSC),经重组腺病毒载体Ad-IL-12转染,构建Ad-IL-12-MSC。方法 Percoll法分离与培养大鼠MSC,流式细胞仪测定细胞表面标志CD29,CD44,CD34。以重组腺病毒载体Ad-IL-12转染MSC,RT-PCR、Western Blott检测转染后IL-12基因mRNA和蛋白的表达。结果分离所得的大鼠MSCs细胞组成比较均一。流式细胞仪检测3种MSCs的表面抗原标志,CD29和CD44,阳性率分别达97.1%和98.2%,而CD34的阳性率仅为4.4%。转染后,Ad-IL-12-MSC细胞IL-12基因在mRNA水平和蛋白质水均有表达。结论 Percoll法分离与培养MSC较为可靠且简单,构建的Ad-IL-12-MSC能成功表达IL-12。
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| 关 键 词： | 腺病毒科 白细胞介素12/遗传学 间质干细胞/代谢 动物 大鼠 |
Expression of IL-12 in MSC infected by recombinant adenoviral vector Ad-IL-12 |
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| WANG Yan-jun,KUI Yu-chuan,YANG Zhi-xiang,YING Chao-hui. Expression of IL-12 in MSC infected by recombinant adenoviral vector Ad-IL-12[J]. Chinese Journal of Misdiagnostics, 2011, 11(3): 516-518 |
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| Authors: | WANG Yan-jun KUI Yu-chuan YANG Zhi-xiang YING Chao-hui |
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| Affiliation: | WANG Yan-jun,KUI Yu-chuan,YANG Zhi-xiang,YING Chao-hui Chest Surgery,Xinha Hospital Affiliated to Dalian Medical University,Dalian,116021,Chin |
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| Abstract: | Objective To isolate the culture of rat MSC,and constructed the MSC which were transfected by recombinant adenoviral vector carrying IL-12 gene.Methods MSC was isolated and cultured by Percoll's method.Flow cytometry to detect surface markers of CD29,CD44 and CD34.Using RT-PCR and Western Blott methods to detect the expression of IL-12 mRNA and its protein in transfected MSC.Results Growth status of MSC separated by Percoll presented dolicho-fusiform shape uniformly.Surface antigen markers of MSC by using F... |
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| Keywords: | Adenoviridae Interleukin-12/genetics Mesenchymal Stem Cells/metabolism Animals Rats |
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