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Complete structure of eclosion hormone of Manduca sexta
Authors:HIROSHI KATAOKA  JORGE P. LI  ALFRED S.T. LUI  STEVEN J. KRAMER  DAVID A. SCHOOLEYS
Abstract:The locations of the three disulfide bonds of eclosion hormone (EH) isolated from Manduca sexta were assigned by sequence analysis of thermolysin fragments and by comparison of a key heterodimeric fragment to regiospecifically synthesized parallel and antiparallel isomers. We elucidated the complete structure of Manduca EH as a 62-residue peptide which has three disulfide bonds between Cys14-Cys38, Cys18-Cys34, and Cys21-Cys49.
Keywords:Bombyx mori  complete primary structure  thermolysin digest  tobacco hornworm
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