淫羊藿甙对体外培养大鼠成骨细胞Cbfa1基因表达的影响

目的观察淫羊藿甙对体外培养大鼠成骨细胞功能及对转录因子核心结合因子a1（Cbfa1）mRNA表达的影响,探讨淫羊藿甙抗骨质疏松的作用机制。方法用酶消化法获得新生（〈24 h）SD大鼠颅盖骨成骨细胞,进行体外原代培养。将不同浓度的淫羊藿甙（1,10,100 ng/ml）分别加入培养液中,观察成骨细胞的形态、增殖和分化功能。用RT-PCR法测定成骨细胞Cbfa1、碱性磷酸酶（ALP）和Ⅰ型胶原（ColⅠ）mRNA表达水平的变化。结果 1、10和100 ng/ml淫羊藿甙均可促进Cbfa1、ALP和ColⅠmRNA表达（P〈0.05）,且以10 ng/ml组作用最显著。结论淫羊藿甙能促进体外培养成骨细胞增殖与分化,通过提高成骨细胞转录因子Cbfa1的基因表达水平诱导骨形成,是其抗骨质疏松作用机制之一。

Effects of icarrin on the mRNA expressions of Cbfa1 in rat osteoblast

Objective To observe the effects of icarrin on the proliferation and differentiation of rat osteoblasts cultured in vitro and to investigate the effects of icarrin on the expressions of Cbfa1 in rat osteoblast. Methods Calvarial osteoblasts were obtained from newborn（24 h） SD rats by trypsin-collagenase digestion method. The culture medium with different icarrin concentrations（1, 10, 100 ng/ml） and the second generation osteoblasts were mixed. The mRNA expressions of Cbfa1、ALP and ColⅠwere detected by semiquantative RT-PCR respectively. Results In comparison with the control group, the mRNA expressions of Cbfa1, ALP and ColⅠ were increased in a dose-dependent manner（P〈0.05） with a maximal effect at the concentration of 10 ng/ml. Conclusion The icarrin had the effects on stimulating the proliferation and differentiation of cultured osteoblast in vitro. The icarrin could induce bone formation by enchancing the basal level of Cbfa1 gene expressions.