Utilization of haem from the haptoglobin-haemoglobin complex by Bacteroides fragilis |
| |
| Affiliation: | 1. Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C., C.I.A.D. A.C., Hermosillo, Sonora, Mexico;2. Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Hermosillo, Sonora, Mexico;3. Coordinación de Ciencias de los Alimentos, C.I.A.D. A.C., Hermosillo, Sonora, Mexico;1. Department of Psychology, City, University of London, London EC1V 0HB, UK;2. Department of Neuroscience, Brown University, Providence, RI 02912, United States of America;3. School of Psychology, The University of Queensland, Brisbane, QLD 4072, Australia;1. Laboratory for Microbiology, School of Dental Medicine, University of Belgrade, Serbia;2. Specialist in Oral Surgery, Department of Medical, Oral and Biotechnological Science, University G. d''Annunzio of Chieti, Italy;3. Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Italy;4. Microbiology Unit, Department of Pharmacy, University of Chieti, Italy;5. Dean of Oral Pathology, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Italy, Ceo of Biomaterials Engineering, Universidad Catolica de San Antonio de Murcia, Spain, Fondazione Villa Serena per la Ricerca, Città S. Angelo, Italy;6. Microbiology and Paediatric Dentistry Unit, Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Via dei Vestini 31, 66013, Italy;1. University of Florence, Department of Chemistry "Ugo Schiff", Via della Lastruccia 13, I-50019 Sesto Fiorentino, Italy;2. University of Florence, NEUROFARBA Dept., Sezione di Scienze Farmaceutiche, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Florence, Italy;3. Centre of Advanced Research in Bionanoconjugates and Biopolymers Department, “Petru Poni” Institute of Macromolecular Chemistry, 700487, Iasi, Romania |
| |
| Abstract: | ![]()
Possession of specialized iron acquisition systems is a prerequisite for the survival of pathogenic bacteria in their host. The purpose of this study was to determine whether Bacteroides fragilis, a clinically important Gram-negative anaerobic bacterium, possesses a specific haem-uptake system. Growth studies indicated that this microorganism can utilize haem from either haemoglobin or haptoglobin-haemoglobin as its sole source of iron. Iron-repressible haem-binding protein complexes (HBP complexes), involved in the uptake of haem from haptoglobin-haemoglobin were detected by means of lithium dodecyl sulfate polyacrylamide gel electrophoresis (LDS-PAGE). Four polypeptides of approximately 60, 58, 49 and 35 kDa, which are part of these HBP complexes, were identified as haem-binding proteins. A 44 kDa iron-repressible outer-membrane protein is needed for a functional HBP complex, but the exact role of this protein in the uptake of haem is still unknown. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
