尘螨变应性鼻炎全血细胞IL-4、IL-13的表达及临床意义

目的探讨IL-4、IL-13在变应性鼻炎发病机制中的作用及IL-4、IL-13拮抗剂治疗变应性鼻炎的临床意义。方法取52例变应性鼻炎（实验组）及25例无过敏性疾病（对照组）患者的外周血，用PMA（phorbol 12-myristate 13-acetate佛波酯）＋inomycin（离子霉素）及标准化尘螨抗原刺激后经细胞内染色，流式细胞仪检测IL-4、IL-13、IFN-γ的表达细胞百分数，ELISA检测血清IL-4、IL-13含量。结果对照组经标准化尘螨变应原刺激后细胞内测出IFN-γ为0．3％-0．4％，但检测不到IL-4与IL-13，经PMA＋inomycin刺激后IFN-γ为5．0％-12．4％、IL-4为0．5％-0．8％、IL-13为0％-0．2％；实验组经标准化尘螨变应原刺激后IFN-γ为0．3％-0．5％、IL-4为0．9％-1．3％、IL-13为0．5％-0．9％，经PMA＋inomycin刺激后IFN-γ为17.3％-24．0％、IL-4为2．1％-3．5％、IL-13为0．8％-2．0％。实验组血清中IL-4含量为（1．768±0．485）pg／ml、IL-13为（5．427±1．263）pg／ml，对照组IL-4与IL-13含量均低于敏感度。结论IL-4、IL-13在变应性鼻炎患者中表达升高，参与了变态反应过程，为临床应用IL-4、IL-13拮抗剂治疗变应性鼻炎提供了依据。

Expression and Clinical Value of IL-4 and IL-13 in Patients with Allergic Rhinitis

Objective To investigate the role of IL-4 and IL-13 in the pathogenesis of allergic rhinitis and the clinical value of antagonist of IL-4 and IL-13 in the treatment of allergic rhinitis. Methods We collected 52 cases of allergic rhinitis. They were allergic to Dermatophagoides pteronyssinus and confirmed by rised specific IgE. Twenty-five cases of normal individual were also collected whose specific IgE are negative as control group. PMA ＋Inomycin and the standard Dermatophagoides pteronyssinus were added into the peripheral blood of all cases. After intracellular staining, the expression of IL-4, IL-13, and IFN-γ detected by FCM. IL-4 and IL-13 in serum of all cases was detected by ELISA. Results In control group, IFN-γ can be tested （0.3%-0.4%） after stimulated by allergen, but IL-4 and IL-13 can not be detectable. After the stimulation of PMA＋inomycin, the levels of IFN-γ, IL-4, and IL-13 were 5.0%- 12.4%, 0.5%-0.8%, and 0%-0.2%. In test group, after stimulated by allergen, the levels of IFN-γ, IL-4, and IL-13 were 0.3%-0.5%, 0.9%-1.3%, and 0.5%-0.9%. After the stimulation of PMA＋ inomycin, the levels of IFN-γ, IL-4, and IL-13 are 17.3%-24.0%, 2.1%-3.5%, and 0.8%-2.0%. The concentration of IL-4 and IL-13 was （1.768 ± 0.485） and （5.427± 1.263） pg/ml in positive stimulated group. They was undotectable in control group. Conclusion IL-4 and IL-13 increas in allergic rhinitis.They may take part in the allergic reactions.