商陆多糖Ⅱ体外对小鼠脾细胞增殖及产生集落刺激因子的影响

用[³H]TdR参入法检测小鼠脾细胞增殖能力及产生集落刺激因子(colony stimulating factor. CSF)含量.证明商陆多糖Ⅱ(PAP-Ⅱ)在31～500 μg·m^-1范围内显著促进小鼠稗细胞增殖。PAP-Ⅱ,31～125 μg·ml^-1可剂量依赖性地促进Con A(1,2.8μg·ml^-1),LPS(3,10,30 μg·ml^-1)诱导的淋巴细胞细咆增殖,随着PAP-Ⅱ剂量加大,对丝裂原诱导的淋巴细胞增殖反呈抑制作用。PAP-Ⅱ。10～500μg·ml^-1呈剂量及时间依赖性地促进脾细胞产生CSF,其最适剂量为100 μg·ml^-1。最佳时间为5 d,提示PAP-Ⅱ能增强免疫及促进造血功能。

EFFECTS OF PHYTOLACCA ACINOSA POLYSACCHARIDES Ⅱ ON LYMPHOCYTE PROLIFERATION AND COLONY STIMULATING FACTOR PRODUCTION FROM MICE SPLENOCYTES IN VITRO

Phytolacca acinosa polysaccharides Ⅱ(PAP-Ⅱ), a kind of polysaccharides isolated from Phytolacca acinosa Roxb with MW 40 kDa, on lymphocyte proliferation and colony stimulating factor (CSF) production from splenocytes in vitro, was studied. The radioactivities of [³H] TdR uptake by lymphocyte and bone marrow cells were used to determine the ability of lymphocyte proliferation and CSF production respectively. PAP-Ⅱ was found to significantly augment splenocyte proliferation in a dose—dependent fashion, and significantly enhance Con A (1, 2. 8 μg·ml^-1) and LPS (3, 10, 30 μg.ml^-1) induced lymphocyte proliferation at concentration of 31～125 μg.ml^-1. As the concentration of PAP-Ⅱ increased, significant suppression of Con A—induced lymphocyte proliferation was observed. Induction of CSF by PAP-Ⅱ from splenocyte was confirmed at the present study. The optimal dosage was 100 μg.ml^-1 and the optimal effect occurred on day 5. These results suggest that PAP-Ⅱ can augment immunological function and enhance hematopoiesis.