气质联用分离测定~(15)N-甘氨酸方法的研究

使用小型气质联用仪测定了血清中１５Ｎ－甘氨酸丰度值，利用代数法分析质谱重叠碎片峰数据，计算得理论值与实际丰度值成线性相关，其相关系数可达０．９９９２。选用健康Ｗｉｓｔｅｒ大鼠一次灌胃１５Ｎ－甘氨酸２０ｍｇ，用该方法测定灌胃前、灌胃后３０ｍｉｎ及１ｈ至７ｈ血清中１５Ｎ－甘氨酸的丰度值，绘制１５Ｎ－甘氨酸含量随时间衰变曲线，结果表明，该方法适用于医学研究中低丰度样品的测定，为稳定同位素丰度测定提供了一个更准确简便的分析方法。

THE STUDY ON THE SEPERATION AND DETERMINATION OF 15 N LABELED GLYCINE BY GC MS

In this paper,the abundance of 15 N labeled glycine was analyzed by a Hewlett Packard 5890Ⅱ gas chromatography 5971A mass spectrometry and calculated using a mathematic method.The calculated results correlated well with the real abundances (r= 0.9992 ).We also used this method to determine 15 N enrichment of plasma in normal rat.The isotope enrichment time decay curve of plasma glycine,following a single oral adminstration of 15 N glycine was obtained.This method can be used in determination of low enrichment samples in medical studies.It established a more accurate,more convenient method for the analysis of stable isotope as a tracer in studying protein metabolism in bodies.