普伐他汀对培养人内皮祖细胞药理作用的影响

目的观察普伐他汀对培养人内皮祖细胞（EPC）数量、增殖、迁移、黏附及一氧化氮（NO）合成能力的影响。方法采用密度梯度离心法分离人外周血单个核细胞，贴壁筛选法培养内皮祖细胞，培养7d后收集细胞并分别加入普伐他汀10μmol／L及100μmol／L，干预48h，免疫组化、荧光显微镜和流式细胞仪鉴定内皮祖细胞，分别观察内皮祖细胞的数量、增殖迁移黏附能力、细胞内皮型一氧化氮合酶（eNOS）、mRNA的表达以及培养液中NO的水平。结果普伐他汀组促进外周血内皮祖细胞扩增，并显著改善外周血内皮祖细胞的黏附、迁移、增殖以及eNOSmRNA表达和NO合成的能力。结论他汀类药物可增加培养人内皮祖细胞数量并改善其功能。

Effects of pravastatin on endothelial progenitor cells in vitro

Objective To investigate the effects of pravastatin on endothelial progenitor cells. Methods Total mononu- clear cells（MNCs） were isolated from human peripheral blood by Fieoll density gradient centrifugation, and cultured in vitro. After 7 days of culture, attached cells were stimulated with pravastatin （10 and 100 μmol/L） for 48 h. The cells were identified by immunohistochemistry and flow cytometry and tested the ability to intake the acLDL. The ability of pro- liferation, migration, adhesion and the content of NO, the levet of endothelial nitric oxide synthase （eNOS） mRNA were tested. Results Compared with control group, the ability of proliferation, migration, adhesion and the Content of NO, the level of endothelial nitric oxide synthase （eNOS） mRNA of pravastatin group increased obviously （P 〈 0.05）. Con- clusion Pravastatin can promote EPC augmentation and enhance its functional activity.