4种不同药材来源郁金饮片中挥发油成分的GC-MS分析

目的:分析4种不同药材来源郁金饮片中挥发油成分,找出其共有及差异成分,为该饮片的合理使用提供依据。方法:采用水蒸气蒸馏法提取挥发油,利用GC-MS对4种不同药材来源郁金饮片挥发油成分进行测定,载气高纯度氦气,进样温度250℃,压力49.5 k Pa,总流量36.0 m L·min~(-1),柱流量1.0 m L·min~(-1),线速度36.1 cm·s~(-1);电离方式电子轰击离子源,电离电压70 e V,离子源温度200℃,接口温度280℃,溶剂延时3.5 min,m/z 40~400。结果:4种不同来源郁金饮片挥发油均检测出了50种成分,其中有3种共有成分,3种郁金共有成分达13种,2种郁金共有成分达15种;4种郁金共有、交互存在的挥发油成分达31种。结论:4种不同药材来源郁金饮片挥发油中存在共有及交互存在的成分。吉马酮可作为温郁金、桂郁金、绿丝郁金的含量测定指标成分;姜黄素、姜黄酮、芳姜黄酮等可作为黄丝郁金的含量测定指标成分。

Analysis of Volatile Oil in Curcumae Radix Pieces from Four Different Sources by GC-MS

Objective: To analyze volatile oil in Curcumae Radix pieces from four different sources and find out the common and different components. Method: The volatile oil in Curcumae Radix pieces from four different sources were extracted by steam distillation and determined by GC-MS,carrier gas was helium,temperature was rose by program,ionization mode was electron bombardment ion source. Result: Fifty components were determined in volatile oil in Curcumae Radix pieces from four different sources and three common components were found in four Curcumae Radix pieces;thirteen common components were found in three Curcumae Radix pieces;fifteen common components were found in two Curcumae Radix pieces;thirty-one components were found in two or more Curcuma Radix pieces,and the proportion was 62%. Conclusion: Curcumae Radix pieces from four different sources have common and interactive components in volatile oil.