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超声提取枸杞叶中总黄酮提取工艺及其不同采收期含量变化研究
引用本文:王汉卿,王文苹,闫津金,隋宏.超声提取枸杞叶中总黄酮提取工艺及其不同采收期含量变化研究[J].中国实验方剂学杂志,2011,17(8):44-47.
作者姓名:王汉卿  王文苹  闫津金  隋宏
作者单位:1. 宁夏医科大学药学院,银川,750004;宁夏回药现代化工程技术研究中心,银川,750004
2. 宁夏医科大学药学院,银川,750004
3. 宁夏医科大学药学院,银川,750004;宁夏医科大学宁夏医药研究所,银川,750004
基金项目:宁夏医科大学青年基金项目
摘    要:目的:优选超声辅助提取宁夏枸杞叶总黄酮的提取工艺并测定其不同采收期总黄酮含量。方法:分别研究乙醇浓度,乙醇用量,超声时间,超声温度4个单因素对提取效果的影响,并设计正交试验,所得结果采用方差分析。利用优选出的最佳超声提取工艺测定比较不同采收期枸杞叶中的总黄酮含量。结果:确定了最佳单因素水平,通过正交试验优选出超声辅助提取枸杞叶总黄酮的最佳工艺条件为乙醇体积分数65%、乙醇用量1:60、超声提取时间35 min、超声温度70℃。结论:通过比较不同采收时期枸杞叶总黄酮的含量,结果为5月中旬含量最高。

关 键 词:宁夏枸杞叶  总黄酮  超声辅助提取  采收期
收稿时间:2010/12/8 0:00:00

Technical Study and Comparison of Total Flavonoids Extracted in Lycium barbarum Leaves of Different Picked Time by Ultrasonography
WANG Han-qing,WANG Wen-ping,YAN Jin-jin and SUI Hong.Technical Study and Comparison of Total Flavonoids Extracted in Lycium barbarum Leaves of Different Picked Time by Ultrasonography[J].China Journal of Experimental Traditional Medical Formulae,2011,17(8):44-47.
Authors:WANG Han-qing  WANG Wen-ping  YAN Jin-jin and SUI Hong
Institution:College of Pharmaceutics, Ningxia Medical University, Yinchuan 750004, China; Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, China;College of Pharmaceutics, Ningxia Medical University, Yinchuan 750004, China; Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, China;College of Pharmaceutics, Ningxia Medical University, Yinchuan 750004, China;College of Pharmaceutics, Ningxia Medical University, Yinchuan 750004, China; Ningxia Research Institute of Medicine & Pharmacy, Ningxia Medical University, Yinchuan 750004, China
Abstract:Objective:To optimally select the extraction technology of total flavonoids of Lycium barbarum leaves by ultrasonography and to determine the content of total flavonoids of L.barbarum leaves of different picked time.Method:The effects of four single-factors(including ethanol concentration,ethanol consumption,ultrasonic extraction time and temperature)on the extraction of total flavonoids of L.barbarum leaves have been studied.And the results of the orthogonal experiment were analyzed by variance analysis.Us...
Keywords:leaves of Lycium barbarum  total flavonoids  ultrasonic extraction  picked time
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