| 复方六月青对肝星状细胞增殖和细胞外基质的影响 |
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| 引用本文： | 张士军,张志伟,陈兆霓,焦杨,李勇文,黄仁彬.复方六月青对肝星状细胞增殖和细胞外基质的影响[J].中国实验方剂学杂志,2011,17(3):135-138. |
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| 作者姓名： | 张士军 张志伟 陈兆霓 焦杨 李勇文 黄仁彬 |
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| 作者单位： | 1. 广西医科大学药理学教研室,南宁,530021
2. 广西中医学院附属瑞康医院,南宁,530011 |
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| 基金项目： | 广西科学研究与技术开发计划项目，广西研究生教育创新计划资助项目 |
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| 摘 要： | 目的:研究复方六月青(CLYQ)对肝星状细胞(HSC)增殖和细胞外基质(ECM)的影响。方法:采用四甲基噻唑蓝(MTT)法检测CLYQ对HSC的毒性。采用酶联免疫吸附实验(ELISA)法检测HSC上清液羟脯氨酸(Hyp)、透明质酸(HA)和金属蛋白酶组织抑制因子-1(TIMP-1)。结果:CLYQ体外对HSC的TC50=6.191 g.L-1,TC0=3.504 g.L-1,CLYQ抑制HSC的增殖。CLYQ各剂量组均可显著降低HSC细胞上清液Hyp,HA,TIMP-1含量(P<0.05,P<0.01),呈现量效反应关系。结论:CLYQ通过抑制HSC的增殖,从而使ECM合成减少,肝纤维化减轻。
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| 关 键 词： | 复方六月青 肝纤维化 肝星状细胞 细胞增殖 细胞外基质 |
| 收稿时间： | 2010/7/19 0:00:00 |
Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells |
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| ZHANG Shi-jun,ZHANG Zhi-wei,CHEN Zhao-ni,JIAO Yang,LI Yong-wen and HUANG Ren-bin.Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells[J].China Journal of Experimental Traditional Medical Formulae,2011,17(3):135-138. |
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| Authors: | ZHANG Shi-jun ZHANG Zhi-wei CHEN Zhao-ni JIAO Yang LI Yong-wen and HUANG Ren-bin |
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| Institution: | Department of Pharmacology, Guangxi Medical University, Nanning 530021, China;Affiliated Ruikang Hospital, Guangxi Traditional Chinese Medicine University, Nanning 530011, China;Department of Pharmacology, Guangxi Medical University, Nanning 530021, China;Department of Pharmacology, Guangxi Medical University, Nanning 530021, China;Department of Pharmacology, Guangxi Medical University, Nanning 530021, China;Department of Pharmacology, Guangxi Medical University, Nanning 530021, China |
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| Abstract: | Objective: To study the influence of CLYQ on proliferation and extracellular matrix(ECM) of hepatic stellate cells(HSC). Method: Cytotoxicity caused by CLYQ was detected by MTT assay. The contents of hydroxyproline (Hyp), hyaluronic acid (HA) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in HSC cell supernate were detected by enzyme linked immunoabsorbent assay (ELISA). Result: TC50 is 6.191 g ·L-1, and TC0 is 3.504 g ·L-1. CLYQ could inhibit the proliferation of HSC. The various concentration group of CLYQ could degrade significantly the contents of Hyp, HA and TIMP-1 in cell supernate (P<0.05, P<0.01), presenting the dose-effect relationship. Conclusion: CLYQ can inhibit the proliferation of HSC, so that reducing the synthesis of ECM and hepatic fibrosis. |
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| Keywords: | Compound Liuyueqing (CLYQ) hepatic fibrosis hepatic stellate cell cell proliferation extracellular matrix |
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