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| RG-14260增强子宫内膜癌细胞化疗敏感性研究 | |
| 引用本文： | 邓守恒,周保锋,喻雄杰,瞿萍,徐华,曹凤军. RG-14260增强子宫内膜癌细胞化疗敏感性研究[J]. 中国实验方剂学杂志, 2011, 17(7): 210-212 |
| 作者姓名： | 邓守恒 周保锋 喻雄杰 瞿萍 徐华 曹凤军 |
| 作者单位： | 1. 湖北医药学院附属人民医院肿瘤中心,湖北,十堰,442000 2. 十堰市妇幼保健院妇产科,湖北,十堰,442000 |
| 基金项目： | 湖北省教育厅基金，湖北医药学院基金 |
| 摘 要： | 目的:研究酪氨酸磷酸化抑制剂RG-14260(RG)单独或联合阿霉素、顺铂及紫杉醇对子宫内膜癌ishikawa细胞增殖的影响,为临床应用RG治疗子宫内膜癌提供理论依据。方法:应用10μmol.L-1RG与各1/2的IC50量的阿霉素(ADM)、顺铂(DDP)及紫杉醇(PTX)联合,MTT法检测细胞增殖抑制率;RT-PCR法检测RG、阿霉素、顺铂及紫杉醇对细胞表皮生长因子受体EGFRmRNA表达影响;Western blot法检测10μmol.L-1RG作用6,12,24 h后细胞p21蛋白表达水平。结果:RG可显著降低细胞EGFR基因mRNA水平,上调p21蛋白表达。结论:RG通过抑制EGFR信号通路,上调p21蛋白表达,增强化疗药物对子宫内膜癌细胞的杀伤作用。 |
| 关 键 词： | 子宫内膜癌 RG-14260 表皮生长因子受体 p21 |
| 收稿时间： | 2010-11-23 |
Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells in vitro |
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| DENG Shou-heng,ZHOU Bao-feng,YU Xiong-jie,QU Ping,XU Hua and CAO Feng-jun. Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells in vitro[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(7): 210-212 | |
| Authors: | DENG Shou-heng ZHOU Bao-feng YU Xiong-jie QU Ping XU Hua CAO Feng-jun |
| Affiliation: | Center of Oncology, Renmin Hospital, Hubei Medical University, Shiyan 442000, China;Department of Gynaecology and Obstetrics, Shiyan Maternal and Child Health Hospital, Shiyan 442000, China;Center of Oncology, Renmin Hospital, Hubei Medical University, Shiyan 442000, China;Center of Oncology, Renmin Hospital, Hubei Medical University, Shiyan 442000, China;Department of Gynaecology and Obstetrics, Shiyan Maternal and Child Health Hospital, Shiyan 442000, China;Center of Oncology, Renmin Hospital, Hubei Medical University, Shiyan 442000, China |
| Abstract: | |
| Keywords: | RG-14260 p21 |
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