内毒素致小鼠SIRS模型建立及两种贯众醇提物对其保护作用的初步观察

目的:通过建立小鼠全身炎症反应综合征(SIRS)模型,研究中药贯众的不同品种对SIRS模型的保护作用。方法:BALB/c小鼠随机分为空白组、模型组和贯众醇提物高、中、低剂量(4.68,2.34,1.17 g·kg-1)组,预防给药7 d后造模,5 h后检测小鼠肛温、呼吸频率、肺湿重/干重比、外周血白细胞计数以及糖脂代谢情况。结果:ip内毒素(LPS)6 mg·kg-1可致小鼠体温明显降低,外周血白细胞数减少,肺组织充血水肿,糖脂代谢水平降低。紫萁贯众和荚果蕨贯众可不同程度地影响LPS所致小鼠的肛温、肺干湿重、血清胆固醇和外周血白细胞数等异常改变,其中紫萁贯众作用更为明显。结论:ip LPS致小鼠SIRS模型成立,紫萁贯众和荚果蕨贯众醇提物对内毒素致小鼠SIRS有一定保护作用。

Protective Effect of Different Extracts of Dryopteris crassirhizoma on Endotoxin Induced Systemic Inflammatory Response Syndrome in Mice

Objective: To study the protective effect of Dryopteris crassirhizoma on systemic inflammatory response syndrome (SIRS) through establishing the mouse model of SIRS. Method: BALB/c mice were randomly devided into several groups,the normal control group,the LPS model group and the different doses of Dryopteris crassirhizoma extract groups(4.68, 2.34, 1.17 g ·kg^-1).The rectal temperature,respiratory rate,wet and dry lung weight ratio,WBC, the level of glucose and lipid metabolism were determined before and after treatment. Result: Intra-peritoneal injection of LPS 6 mg ·kg^-1 could significantly lower the body temperature,reduce WBC,make the lung congestion and edema,reduce the level of glucose and lipid metabolism. Osmunda japonica and Matteuccia struthiopteris could raise the low temperature and reduction of WBC caused by LPS,improve the abnormal serum choleterol. Conclusion: O. japonica and M. struthiopteris have marked effect in protecting LPS caused SIRS.