安心颗粒对心衰大鼠氧化应激和心肌细胞凋亡的影响

目的:观察安心颗粒对慢性心力衰竭模型大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平及心肌细胞凋亡的影响。方法:将50只雌性SD大鼠随机分为正常对照组(A组)、模型组(B组)、卡托普利组(C组)、安心颗粒低剂量组(D组)、安心颗粒高剂量组(E组),每组10只。采用阿霉素ip造模,每周1次,连续6周。造模同时,C～E组分别按6.25 mg.kg-1.d-1,0.8,1.6 g.kg-1.d-1剂量ig给药。造模结束后,分别测定各组血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平,左室重量指数(LVMI)及心肌细胞凋亡指数(AI)。结果:模型组血清MDA水平、LVMI及AI均显著性升高(P<0.01),血清SOD活性显著下降(P<0.01);安心颗粒低、高剂量组均可显著性降低模型大鼠血清MDA水平、LVMI及AI(P<0.05或P<0.01),提高血清SOD活性(P<0.01)。结论:安心颗粒可通过调控氧化应激、抑制心肌细胞凋亡,延缓心肌重构,防治心力衰竭。

Effects of Anxin Granules on Oxidative Stress in Serum andMyocardial Cell Apoptosis in Heart Failure Rats

Objective: To observe the effects of Anxin Granules (AXG) on the levels of serum superoxide dismutase (SOD) and malondialdehyde(MDA) and myocardial cell apoptosis in rats with chronic heart failure (CHF) ,and to explore the possible therapeutic mechanism. Method: Fifty female SD rats were randomly divided into five groups:normal control group (A),model group (B),captopril group (C),low-dose AXG group (D) and high-dose AXG group (E).CHF models were established by intraperitoneal injection of small-dose of adriamycin,once per week for continuous 6 weeks.Meanwhile,group C was injected captopril (6.25 mg ·kg^-1·d^-1); groups D and E received low-dose AXG (0.8 g ·kg^-1·d^-1) and high-dose AXG (1.6 g ·kg^-1·d^-1) ig respectively.After 6 weeks,serum levels of SOD, MDA and the left ventricular mass index (LVMI) of different groups were detected. Myocardial cells apoptosis was tested by TUNEL. Apoptosis index (AI) was counted. Result: Compared to group A,the serum levels of MDA ,and LVMI,and AI in cardiac muscle were markedly increased in the model group (P<0.01),the activity of SOD in serum was significantly decreased (P<0.01) in rats model with CHF. AXG decreased the serum levels of MDA,LVMI and AI compared with the model group (P<0.05 or P<0.01).And the level of SOD was ascended markedly. Conclusion : AXG inhibit apoptosis and anti-oxidative stress.