左卡尼汀联合西地那非对雄性糖尿病大鼠生殖内分泌功能的保护作用

目的:初步探讨左卡尼汀与西地那非片在保护糖尿病(DM)大鼠生殖内分泌功能中的作用。方法:40只体重为200～230 g的雄性SD大鼠随机均分为5组:A组为正常对照组,B组为DM大鼠模型组,C组为DM大鼠给予西地那非[5 mg/(kg.d)]治疗组,D组为DM大鼠给予左卡尼汀[300 mg/(kg.d)]治疗组,E组为DM大鼠给予左卡尼汀[300 mg/(kg.d)]联合西地那非[5 mg/(kg.d)]治疗组。各组大鼠治疗6周后分别进行血睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)的检测。结果:糖尿病大鼠治疗6周后,血清T,FSH、LH含量,A组:T为(25.25±2.67)nmol/L,FSH为(5.78±0.61)IU/L,LH为(625.21±43.45)ng/L;B组:T为(9.63±1.71)nmol/L,FSH为(1.98±0.42)IU/L,LH为(479.89±27.62)ng/L:C组:T为(18.98±3.07)nmol/L,FSH为(5.08±0.33)IU/L,LH为(586.57±31.72)ng/L;D组:T为(16.18±2.65)nmol/L,FSH为(4.63±0.30)IU/L;LH为(540.78±25.52)ng/L;E组:T为(23.65±2.66)nmol/L,FSH为(5.59±0.48)IU/L,LH为(621.53±36.40)ng/L。各组血清T,FSH、LH含量之间比较,B组均显著低于A、C、D、E组(P均<0.01);C、D组均显著低于A、E组(P均<0.05),而C、D组之间比较,差异均无显著性(P>0.05);E组与A组比较,差异也均无显著性(P>0.05)。结论:西地那非片与左卡尼汀灌胃6周后均可增加DM大鼠血清T、FSH、LH的水平,而两者联合用药时,T、FSH、LH增加水平比单独用药更明显,显示联合用药在保护雄性DM大鼠生殖内分泌功能方面效果更佳。

Protective effect of L-carnitine combined with sildenafil on the reproductive endocrine function of diabetic male rats

Objective: To investigate the protective effect of L-carnitine(LC) combined with sildenafil on the reproductive endocrine function of male rats with diabetes mellitus(DM).Methods: A total of 40 male SD rats were randomly divided into five groups,group A taken as normal controls,and groups B,C,D and E made into DM models by injection of streptozotocin at 65 mg/kg.Then the rats in groups A and B were treated with normal saline,C with sildenafil at 5 mg per kg per d,D with LC at 300 mg per kg per d,and E with sildenafil at 5 mg per kg per d plus LC at 300 mg per kg per d,all via gastric gavage for 6 weeks,followed by determination of the levels of testosterone(T),follicle-stimulating hormone(FSH) and luteinizing hormone(LH) in the serum of the rats.Results: After 6 weeks of treatment,the T,FSH and LH levels were(25.25±2.67) nmol/L,(5.78±0.61) IU/L and(625.21±43.45) ng/L in group A,(9.63±1.71) nmol/L,(1.98±0.42) IU/L and(479.89±27.62) ng/L in group B,(18.98±3.07) nmol/L,(5.08±0.33) IU/L and(586.57±31.72) ng/L in group C,(16.18±2.65) nmol/L,(4.63±0.30) IU/L and(540.78±25.52) ng/L in group D,and(23.65±2.66) nmol/L,(5.59±0.48) IU/L and(621.53±36.40) ng/L in group E.The three parameters were significantly lower in B than in the other four groups(P<0.01),and so were they in C and D than in A and E(P<0.05),but showed no significant differences either between C and D(P>0.05) or between A and E(P>0.05).Conclusion: Six-week medication of either sildenafil or LC alone could increase the levels of T,FSH and LH in the serum of DM rats,but the combination of the two had an even more obvious increasing effect,which indicates a still better protective effect on the reproductive endocrine function of diabetic male rats.