间接免疫荧光技术应用于狂犬病抗体IgG检测研究

目的：研究间接免疫荧光技术在狂犬病抗体中的应用。方法：由本中心研制狂犬抗原片和荧光血清,比较间接免疫荧光技术与酶标方法的效果。结果：间接免疫荧光技术检测狂犬病抗体特异性、稳定性良好、变异系数CV＝4．9％。间接免疫荧光技术与ELISA同时检测,间接免疫荧光法阳性率为82．89％,ELISA法阳性率为40．48％。结论：间接免疫荧光法测定狂犬病抗体特异性、稳定性好。狂犬疫苗免疫后应用该技术检测抗体,确保免疫效果。

Research on the application of indirect immunofluorescent antibody technique to detect rabies antibody IgG

[Objective] To study the application of indirect immunofluorescent antibody technique to detect rabies antibody IgG (refers to " IFAT" as follows) . [Methods] The effect of IFAT and enzyme labeling method was compared using rabies antigenic pieces and fluorescent serum made in our center. [Results] IFAT was found with good stability with a coefficient of variation CV of 4.9% . When both IFAT and ELBA were adopted, the positive rate of IFAT was 82.89% , and ELBA 40.78% . [Conclusion] Using IFAT to detect the specificity of rabies antibody achieved good stability. The effect of immunization can be assured by using this technique to detect antibody after accepting rabies vaccine.