SRS-82细胞株的无细胞提取液的致白血病活性研究

小鼠SRS-82细胞株的电镜观察表明,其超微结构显示淋巴细胞的特点,在胞质扩大的囊泡内见到池内A型病毒颗粒。SRS-82株的无细胞提取液注射昆明种新生乳鼠24只,其中15只发生白血病,诱发率为62.5%。14只为淋巴细胞白血病,1只为粒细胞白血病。SRS-82株培养上清液的超速离心沉淀物混悬液,注入16只昆明种新生乳鼠,有4只小鼠诱发淋巴细胞白血病。上述研究结果表明。SRS-82株的无细胞提取液和其培养上清液的离心沉淀物皆具有致白血病活性。此外,对病毒与诱发不同的白血病类型之间的关系进行了简要的讨论。

Studies on leukemogenic activity of cell-free extract of mouse SRS-82 cell line

Ultrastructural studies of SRS-82 cell line demonstrated certain morphologic characteristics of lymphocytes and the presence of intracistenal type A virus in the cytoplasm. Inoculation of cell-free extract from SRS-82 cell line into 24 newborn mice of Kunmin strain, leukemias were induced in 15 mice (62.5%). Among them 14 are lymphocytic (14/15) and only one is myelocytic (1/15). The average induction period was 169 days. Furthermore, on transfer of ultracentifugation precipitate suspension of SRS-82 culture supernate into 16 newborn mice of Kunmin strain, the incidence of leukemia was 250% (all were lymphocytic). The results showed that both the cell-free extract and culture supernate from mouse SRS-82 cell line were leukemogenic. The relationship between the virus and the type of leukemia induced remains to be studied.