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Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei |
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| Authors: | Steffen Dietzel Anna Jauch Dirk Kienle Guoquiong Qu Heidi Holtgreve-Grez Roland Eils Christian Munkel Michael Bittner Paul S. Meltzer Jeffrey M. Trent Thomas Cremer |
| Affiliation: | (1) Center of Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany;(2) Institute of Human Genetics, University of Heidelberg Heidelberg, Germany;(3) Interdisciplinary Center of Scientific Computing, University of Heidelberg, Heidelberg, Germany;(4) Division of Biophysics of Macromolecules, Deutsches Krebsforschungszentrum, Heidelberg, Germany;(5) Laboratory of Cancer Genetics, National Human Genome Research Institute, National Institutes of Health, Bethesda, USA |
| Abstract: | Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding. |
| Keywords: | chromosome arms chromosome structure chromosome territories confocal microscopy fluorescence in situ hybridization nuclear organization random walk/giant loop model |
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