Glutathione metabolizing enzyme activities in human thyroid |
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| Authors: | G Del Boccio R Casaccia A Aceto E Casalone P De Remigis C Di Ilio |
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| Affiliation: | 1. Institutes of Biochemical Sciences, Faculty of Medicine, University of “G. D''Annunzio” 66100 Chiety, Italy;1. Institutes of Internal Medicine, Faculty of Medicine, University of “G. D''Annunzio” 66100 Chiety, Italy;1. Department of Pharmacology, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016 Shenyang, PR China;2. Benxi Institute of Pharmaceutical Research, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016 Shenyang, PR China;3. Key Laboratory of Structure-Based Drugs Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016 Shenyang, PR China;1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China;2. Yangzhou Integrated Experimental Station, National Industry Technology Research System of Staple Freshwater Fish, Jiangsu 225104, China;3. Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA;1. Institute for Biological Research, “Siniša Stanković”, University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia;2. Institut für Klinische Chemie und Klinische Pharmakologie, Universitätsklinikum Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany;3. Institut für Experimentelle Endokrinologie, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany;4. Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia;1. National School of Public Health, Oswaldo Cruz Foundation, CEP: 21041-210 Rio de Janeiro, Brazil;2. University of Serra Gaúcha, CEP: 95020-472 Caxias do Sul, Brazil;3. Biomedical Research Centre Network for Epidemiology and Public Health (CIBERESP), Spain |
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| Abstract: | ![]()
Glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. It was deduced, from measurements of glutathione peroxidase activity with both H202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. The absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel filtration experiments. An interindividual variation of about 28-fold was found measuring glutathione transferase activity with 1-chloro-2,4-dinitrobenzene. Subjecting a fraction of human thyroid cytosols partially purified by G-100 Sephadex column to isoelectricfocusing run, a single peak of glutathione transferase activity centered at pH 4.6 was obtained. An adequate level of glutathione reductase and gamma-glutamyl transpeptidase activities was also found in all specimens investigated. |
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