曲靖市某医院女性患者人乳头瘤病毒检测及基因分型结果分析

目的了解本院女性患者人乳头瘤病毒(HPV)感染情况及HPV基因型分布情况,为临床诊治HPV感染提供依据。方法选取2016年1月1日—2018年11月30日在曲靖市第二人民医院进行HPV检测的20岁及以上女性患者作为研究对象,采用聚合酶链反应(PCR)-反向点杂交技术定性检测HPV并进行基因分型。统计检测结果,分析HPV各年龄段感染率、人群分布以及基因型分布。结果共纳入3 221例受检者,486例患者感染HPV,感染率15.09%;其中408例感染高危型,感染率12.67%。HPV基因分型为16型的患者数量最多,为106例(占21.81%);73型最少,为1例(占0.20%)。体检人群的HPV感染率明显低于门诊和住院患者(10.12%比18.36%、16.25%,P<0.05)。不同年龄段HPV感染率差异不大,≥60岁年龄段人群的感染率最高(21.64%),与30～39岁、40～49岁年龄段感染率(分别为14.13%和14.46%)有明显差异(P<0.05),其他年龄段感染率均无明显差异(均P>0.05)。结论本院体检人群的HPV感染率最低;老年人群的HPV感染率最高;高危型HPV16型、52型、58型以及低危型HPV40型、44型是曲靖市女性HPV感染的主要基因分型。

Analysis of human papilloma virus detection and genotyping results of female patients in a hospital in Qujing City

Objective To understand human papilloma virus(HPV) infection and distribution of HPV genotypes in female patients in our hospital and provide evidence for clinical diagnosis and treatment of HPV infection. Methods From January 1, 2016 to November 30, 2018, female patients aged ≥20 with HPV testing in the Second People’s Hospital of Qujing City were selected as research objects. The polymerase chain reaction(PCR)-reverse dot hybridization technique was used to qualitatively detect the HPV, and HPV genotyping was carried out for the patients. The test results were calculated, and the HPV infection rates, population distribution and the genotype distribution in patients’ various age groups were analyzed. Results In 3 221 female patients aged 20 and over, there were 486 patients infected with HPV, the infection rate being 15.09%, and 408 HPV patients were of high-risk type, the incidence being 12.67%. Analysis of HPV genotyping showed the largest number of patients with 16 genotype(106 cases, 21.81%), and the least number of patients with 73 genotype(1 case, 0.20%). HPV infection rate in physical examination population was significantly lower than the rates in outpatients and inpatients(10.12% vs. 18.36%, 16.25%, both P < 0.05). The differences in HPV infection rates among different age groups were not great, the infection rate of ≥60 years old was the highest(21.64%), and the infection rate was significantly different from the rates in age of 30-39 years old and 40-49 years old groups(14.13%, 14.46%, both P < 0.05), but no significant differences in infection rate among other age groups(all P > 0.05). Conclusion The rate of HPV infection was the lowest among the population undergoing physical examination in our hospital;among the various age groups of HPV patients, the elderly group has the highest rate of HPV infection, the main genotypes of HPV infection in high-risk female patients were HPV16, 52, 58 types, while those of the low-risk ones were HPV40 and 44 in Qujing City.