清醒Beagle犬呼吸和循环系统功能监测模型建立及生物学特性分析

目的:应用植入式遥测技术,建立Beagle犬清醒动物呼吸和循环系统功能检测模型,应用遥测技术对Beagle犬生理参数进行长时程采集和分析,为临床前药物安全性评价试验提供重要的生理基础参考值。方法:通过外科手术,在Beagle犬体内埋植TL11M3-D70-PCTP遥测传感器,以DSI遥测生理信号采集系统连续记录并分析Beagle犬在无束缚条件下24 h生理信号,分析其昼夜节律,并以特非那定作为阳性药物进行验证。结果:成功建立了Beagle犬植入式清醒动物模型,Beagle犬的生理活动具有明显的昼夜节律性,早上09:00左右是多项生理指标的最高点,特非那定能够明显延长Beagle犬的QTc间期。结论:试验获得了本中心GLP试验室饲养条件下动物的基础值,可为其在药物临床前试验中的应用提供重要的参考数据。

Establishment and parameter analysis of a system for simultaneously monitoring cardiovascular and respiratory function in conscious Beagle dogs

Objective: To establish the Beagle dog model with implanted telemetry transmitter,and analyze the parameters of cardiovascular and respiratory systems in the conscious Beagle dogs.Methods: We implanted the telemetry TL11M3-D70-PCTP transmitters to the dogs.The 24-h parameters of cardiovascular and respiratory systems in the conscious telemetered dogs were recorded and analyzed using Dataquest ART 4.8 system.Terfenadine 30 mg·kg-1 was used as a positive control.Results: Telemetry transmitters were successfully implanted.A circadian rhythm was obvious in the parameters from the conscious dogs;at 09:00AM,most parameters reached their peaks.Terfenadine prolonged QTc intervals in Beagle dogs.Conclusion: The conscious and telemetered Beagle dogs can be used for simultaneously assessing cardiovascular and respiratory changes for a longer term in the studies of safety pharmacology.