锰烟尘对男工血脂质过氧化和抗氧化酶的影响

目的研究长期高浓度锰烟尘暴露对男工体内脂质过氧化和抗氧化酶的影响。方法用分光光度法测定59名接触锰烟尘男工和59名对照男工的红细胞血红蛋白(Hb)、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和血清谷胱甘肽S-转移酶(GSH-ST)活力。结果作业环境空气中MnO2平均浓度为0.75 mg/m3(0.19~1.60 mg/m3)时,接锰作业组红细胞MDA含量、SOD、CAT、GSH-Px活力与对照组比较,差异无显著性。分层分析显示,接触锰男工红细胞SOD、CAT、GSH-Px的酶活力随接触锰工龄的增加而升高,接触锰工龄>10 a组,SOD、CAT、GSH-Px酶活力均降至对照组水平。结论接触高浓度的锰<10 a,可使体内抗氧化酶活力应激性增强,而脂质过氧化物保持不变。但接触高浓度锰>10 a,可导致男工血脂质过氧化物增加及抗氧化酶活力降低,对机体产生损害作用。

Effects of Manganese Fume and Dust on Lipid Peroxidation and Antioxidant Enzymes in Blood of Exposed Male Workers

Objective To study the effects of long-term manganese fume and dust exposure on lipid peroxidation and antioxidant enzymes in exposed workers.Methods The concentration of malondialdehyde(MDA),activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)in erythrocyte,and the activities of glutathione s-transferases(GSH-ST)in plasma were measured in 59 exposed workers and 59 control workers by spectrophotometer.Results When the average concentration of MnO_2 in workplaces was 0.75 mg/m~3(0.19~1.60 mg/m~3),no significant change of all indicators was found.The activities of SOD,CAT,and GSH-Px in erythrocyte increased with the increase of working ages.However,in the group of >10a exposure,all the activities of protective enzymes in erythrocyte lowered to the level of the control.Conclusions It suggests that when the exposure<10a,it would strengthen the activities of antioxidant enzymes and keep the lipid peroxidation product remained unchanged.However,when the exposure >10a,it might cause the lipid peroxidation product to increase and activities of antioxidant enzymes to decrease causing damage to the body.